CiPerGenesis, A Mutagenesis Approach that Produces Small Libraries of Circularly Permuted Proteins Randomly Opened at a Focused Region: Testing on the Green Fluorescent Protein [0.03%]
一种产生环状变构蛋白质的小型随机文库的诱变方法——在绿色荧光蛋白上进行试验：CiPerGenesis
Paul Gaytán,Abigail Roldán-Salgado,Jorge A Yáñez et al.
Paul Gaytán et al.
Circularly permuted proteins (cpPs) represent a novel type of mutant proteins with original termini that are covalently linked through a peptide connector and opened at any other place of the polypeptide backbone to create new ends. cpPs ar...
Constrained Combinatorial Libraries of Gp2 Proteins Enhance Discovery of PD-L1 Binders [0.03%]
约束组合文库中的Gp2蛋白可增强PD-L1结合剂的发现工作
Max A Kruziki,Vidur Sarma,Benjamin J Hackel
Max A Kruziki
Engineered protein ligands are used for molecular therapy, diagnostics, and industrial biotechnology. The Gp2 domain is a 45-amino acid scaffold that has been evolved for specific, high-affinity binding to multiple targets by diversificatio...
Out-of-Plane Ionic Conductivity Measurement Configuration for High-Throughput Experiments [0.03%]
高通量实验的平面外离子电导测量配置
Ruiyun Huang,Chris J Kucharczyk,Yangang Liang et al.
Ruiyun Huang et al.
An approach for measuring conductivity of thin-film electrolytes in an out-of-plane configuration, amenable to high-throughput experimentation, is presented. A comprehensive analysis of the geometric requirements for success is performed. U...
Yanbing Han,Bethany Matthews,Dennice Roberts et al.
Yanbing Han et al.
High-throughput synthesis and characterization methods can significantly accelerate the rate of experimental research. For physical vapor deposition (PVD), these methods include combinatorial sputtering with intentional gradients of metal/m...
Protein-Induced Fluorescence Enhancement Based Detection of Plasmodium falciparum Glutamate Dehydrogenase Using Carbon Dot Coupled Specific Aptamer [0.03%]
基于碳点耦合特异性适配体的疟疾标志物蛋白荧光增强型检测法研究蛋白质诱导的荧光增强型疟原虫谷氨酸脱氢酶检测方法研究
Naveen Kumar Singh,Babina Chakma,Priyamvada Jain et al.
Naveen Kumar Singh et al.
A novel 90-mer long ssDNA aptamer (NG3) covering a 40-mer random region targeting Plasmodium falciparum glutamate dehydrogenase ( PfGDH) developed through systematic evolution of ligands by exponential enrichment (SELEX) technique. The bind...
Jonas Petersen,Katrine E Christensen,Mathias T Nielsen et al.
Jonas Petersen et al.
We herein present a broadly useful method for the chemoselective modification of a wide range of tryptophan-containing peptides. Exposing a tryptophan-containing peptide to 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) resulted in a selec...
A Phenotypic Cell-Binding Screen Identifies a Novel Compound Targeting Triple-Negative Breast Cancer [0.03%]
一种表型细胞结合筛选方法发现了一种针对三阴性乳腺癌的新化合物
Luxi Chen,Chao Long,Jonghae Youn et al.
Luxi Chen et al.
We describe a "phenotypic cell-binding screen" by which therapeutic candidate targeting cancer cells of a particular phenotype can be isolated without knowledge of drug targets. Chemical library beads are incubated with cancer cells of the ...
How Transparent Oxides Gain Some Color: Discovery of a CeNiO3 Reduced Bandgap Phase As an Absorber for Photovoltaics [0.03%]
透明氧化物如何获得一些颜色：CeNiO3 缺电子能隙相作为光伏吸收剂的发现
Hannah-Noa Barad,David A Keller,Kevin J Rietwyk et al.
Hannah-Noa Barad et al.
In this work, we describe the formation of a reduced bandgap CeNiO3 phase, which, to our knowledge, has not been previously reported, and we show how it is utilized as an absorber layer in a photovoltaic cell. The CeNiO3 phase is prepared b...
Mark L G Borst,Cécile M J Ouairy,Sander C Fokkema et al.
Mark L G Borst et al.
The design and synthesis of three novel polycyclic scaffolds containing sulfoximines are presented in this work, which exemplify that sulfoximines represent a real opportunity for the discovery of new drug candidates. Additionally, the stru...
Current Parallel Solid-Phase Synthesis of Drug-like Oxadiazole and Thiadiazole Derivatives for Combinatorial Chemistry [0.03%]
用于组合化学的药物类氧化氮杂环化合物和噻二唑衍生物的并行固相合成法研究
Aizhan Abdildinova,Young-Dae Gong
Aizhan Abdildinova
Solid-phase organic synthesis is a powerful tool in the synthesis of small organic molecules and building of libraries of compounds for drug discovery. Heterocyclic compounds are important components of the drug discovery field as well and ...