Single-step Replacement of an Unreactive C-H Bond by a C-S Bond Using Polysulfide as the Direct Sulfur Source in Anaerobic Ergothioneine Biosynthesis [0.03%]
无氧环境下利用多硫化物直接供硫一步取代惰性C-H键合成麦角硫因中的C-S键
Ronghai Cheng,Lian Wu,Rui Lai et al.
Ronghai Cheng et al.
Ergothioneine, a natural longevity vitamin and antioxidant, is a thiol-histidine derivative. Recently, two types of biosynthetic pathways were reported. In the aerobic ergothioneine biosynthesis, a non-heme iron enzyme incorporates a sulfox...
Aldehyde Production in Crude Lysate- and Whole Cell-Based Biotransformation Using a Noncanonical Redox Cofactor System [0.03%]
基于粗酶液和全细胞生物转化的醛类产物的生成及非经典辅因子体系的研究
Kelly N Richardson,William B Black,Han Li
Kelly N Richardson
It is challenging to biosynthesize industrially important aldehydes, which are readily consumed by the numerous alcohol dehydrogenases (ADHs) in cells. In this work, we demonstrate that a nicotinamide mononucleotide (NMN+)-dependent redox c...
Enantioselective, Aerobic Copper-Catalyzed Intramolecular Carboamination and Carboetherification of Unactivated Alkenes [0.03%]
未活化烯烃的对映选择性、需氧铜催化分子内卡宾氮
Tomasz Wdowik,Samuel L Galster,Raul L L Carmo et al.
Tomasz Wdowik et al.
Reduction of waste is an important goal of modern organic synthesis. We report herein oxidase reactivity for enantioselective intramolecular copper-catalyzed alkene carboamination and carboetherification reactions where previously used stoi...
Conor E Brigham,Christian A Malapit,Naish Lalloo et al.
Conor E Brigham et al.
This report describes the development of a nickel-catalyzed decarbonylative reaction for the synthesis of fluoroalkyl thioethers (RFSR) from the corresponding thioesters. Readily available, inexpensive, and stable fluoroalkyl carboxylic aci...
Editing Domain Motions Preorganize the Synthetic Active Site of Prolyl-tRNA Synthetase [0.03%]
编辑作用域运动预组织脯氨酰-tRNA合成酶的合成活性位点
Quin H Hu,Murphi T Williams,Irina Shulgina et al.
Quin H Hu et al.
Prolyl-tRNA synthetases (ProRSs) catalyze the covalent attachment of proline onto cognate tRNAs, an indispensable step for protein synthesis in all living organisms. ProRSs are modular enzymes and the "prokaryotic-like" ProRSs are distingui...
A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site [0.03%]
一种基于生长的高通量筛选平台能够实现对4-羟基苯甲酸羟化酶活性位点的重塑
Sarah Maxel,Derek Aspacio,Edward King et al.
Sarah Maxel et al.
We report an aerobic, growth-based selection platform founded on NADP(H) redox balance restoration in Escherichia coli, and we demonstrate its application in the high-throughput evolution of an oxygenase. A single round of selection followe...
Kinetics and Mechanism of Plasmon-Driven Dehalogenation Reaction of Brominated Purine Nucleobases on Ag and Au [0.03%]
Ag和Au表面等离激元驱动的嘌呤核苷酸衍生物分子脱卤化反应的动力学及机制研究
Anushree Dutta,Robin Schürmann,Sergio Kogikoski Jr et al.
Anushree Dutta et al.
Plasmon-driven photocatalysis is an emerging and promising application of noble metal nanoparticles (NPs). An understanding of the fundamental aspects of plasmon interaction with molecules and factors controlling their reaction rate in a he...
Revealing the CO Coverage-Driven C-C Coupling Mechanism for Electrochemical CO2 Reduction on Cu2O Nanocubes via Operando Raman Spectroscopy [0.03%]
基于operando拉曼光谱的Cu2O纳米立方体电化学二氧化碳还原的CO覆盖驱动C-C耦合机制研究
Chao Zhan,Federico Dattila,Clara Rettenmaier et al.
Chao Zhan et al.
Electrochemical reduction of carbon dioxide (CO2RR) is an attractive route to close the carbon cycle and potentially turn CO2 into valuable chemicals and fuels. However, the highly selective generation of multicarbon products remains a chal...
Aryl Fluoride Activation Through Palladium-Magnesium Bimetallic Cooperation: A Mechanistic and Computational Study [0.03%]
钯-镁双金属合作活化芳基氟的研究：机制和计算研究
Chen Wu,Samuel P McCollom,Zhipeng Zheng et al.
Chen Wu et al.
Herein is described a mechanistic study of a palladium-catalyzed cross-coupling of aryl Grignard reagents to fluoroarenes that proceeds via a low-energy heterobimetallic oxidative addition pathway. Traditional oxidative additions of aryl ch...
Multiscale Simulations of SARS-CoV-2 3CL Protease Inhibition with Aldehyde Derivatives. Role of Protein and Inhibitor Conformational Changes in the Reaction Mechanism [0.03%]
SARS-CoV-2 3CL蛋白酶与亚胺类抑制剂的多尺度模拟研究：蛋白质和抑制剂构象变化对反应机理的影响
Carlos A Ramos-Guzmán,J Javier Ruiz-Pernía,Iñaki Tuñón
Carlos A Ramos-Guzmán
We here investigate the mechanism of SARS-CoV-2 3CL protease inhibition by one of the most promising families of inhibitors, those containing an aldehyde group as a warhead. These compounds are covalent inhibitors that inactivate the protea...