A Simplified In Vitro Teratoma Assay for Pluripotent Stem Cells Injected Into Rodent Fetal Organs [0.03%]
一种简化的多能干细胞注射到啮鼠胚胎器官的畸胎瘤试验方法
Shigeo Masuda,Takashi Yokoo,Naomi Sugimoto et al.
Shigeo Masuda et al.
Teratoma formation assays are established methods for evaluating the pluripotency of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Teratoma formation in immunodeficient mice takes approximately 2 months. Here, we have ...
Generation of Mouse STO Feeder Cell Lines That Confer Resistance to Several Types of Selective Drugs [0.03%]
生成具有多种类型选择性药物抗性的小鼠STO饲养细胞系
Issei Saitoh,Masahiro Sato,Yoko Iwase et al.
Issei Saitoh et al.
Feeder cells are generally required for establishment and maintenance of embryonic stem (ES)/induced pluripotent stem (iPS) cells. Increased demands for generation of those cells carrying various types of vectors (i.e., KO vectors and trans...
Yoshitaka Miyamoto,Hirofumi Noguchi,Hiroshi Yukawa et al.
Yoshitaka Miyamoto et al.
Induced pluripotent stem (iPS) cells have attracted attention as a promising cell source for medical treatment that could replace marrow stromal cells (MSCs) and adipose tissue-derived stem cells (ASCs). These pluripotent cells can be induc...
Clinical Significance of the Pharmacological Efficacy of Tacrolimus Estimated by the Lymphocyte Immunosuppressant Sensitivity Test (LIST) Before and After Renal Transplantation [0.03%]
肾移植前后的淋巴细胞免疫抑制剂敏感性试验(LIST)阳性对Tacrolimus药理效应及临床意义的影响分析
Kentaro Sugiyama,Kazuya Isogai,Akira Toyama et al.
Kentaro Sugiyama et al.
The lymphocyte immunosuppressant sensitivity test (LIST) with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay procedure can predict the pharmacological efficacy of immunosuppressive agents. A previous study repo...
Higher Sensitivity of Peripheral Blood Lymphocytes to Endogenous Glucocorticoid in Renal Transplant Recipients Treated With Tacrolimus, as Compared to Those Treated With Cyclosporine [0.03%]
与环孢素治疗相比，他克莫司治疗的肾移植受者外周血淋巴细胞对内源性糖皮质激素敏感性更高
Gulimire Muhetaer,Hironori Takeuchi,Sogo Akizuki et al.
Gulimire Muhetaer et al.
Lymphocyte sensitivity to endogenous glucocorticoid cortisol could be a biological marker for safe reduction and withdrawal of steroids in renal transplant recipients. We compared peripheral lymphocyte sensitivity with cortisol between tran...
Eicosapentenoic Acid Attenuates Allograft Rejection in an HLA-B27/EGFP Transgenic Rat Cardiac Transplantation Model [0.03%]
依克萨普塔诺酸通过转基因HIA-B27/EGFP大鼠心脏移植模型抑制移植物抗宿主反应
Zhong Liu,Naoyuki Hatayama,Lin Xie et al.
Zhong Liu et al.
The development of an animal model bearing definite antigens is important to facilitate the evaluation and modulation of specific allo-antigen responses after transplantation. In the present study, heterotopic cardiac transplantation was pe...
Differentiation of Mouse Pancreatic Stem Cells Into Insulin-Producing Cells by Recombinant Sendai Virus-Mediated Gene Transfer Technology [0.03%]
利用重组仙台病毒介导的基因转移技术将小鼠胰腺干细胞分化为产生胰岛素细胞
Hiroshi Yukawa,Hirofumi Noguchi,Koichi Oishi et al.
Hiroshi Yukawa et al.
Islet transplantation, including β-cells, has proven to be effective for diabetes in many recent studies; however, this treatment strategy requires sufficient organ donors. One attractive approach for the generation of β-cells is to utili...
Novel Positive-Charged Nanoparticles for Efficient Magnetic Resonance Imaging of Islet Transplantation [0.03%]
高效磁共振成像用新型阳性纳米颗粒应用于胰岛移植
Koichi Oishi,Hirofumi Noguchi,Hiroaki Saito et al.
Koichi Oishi et al.
Significant graft loss immediately after islet transplantation occurs due to immunological and nonimmunological events. Magnetic resonance imaging (MRI) is an attractive potential tool for monitoring islet mass in vivo. Although an efficien...
A Combined Continuous Density/Osmolality Gradient for Supplemental Purification of Human Islets [0.03%]
人胰岛细胞的联合连续密度/渗透梯度法纯化技术
Hirofumi Noguchi,Bashoo Naziruddin,Masayuki Shimoda et al.
Hirofumi Noguchi et al.
For islet transplantation, islet purification minimizes the risks associated with islet infusion through the portal vein. However, islet purification may result in decreased numbers of islets recovered from digested tissue. In this study, w...
Hepatocyte Is a Sole Cell Type Responsible for the Production of Coagulation Factor IX In Vivo [0.03%]
肝细胞是体内产生凝血因子IX的唯一细胞类型
Kohei Tatsumi,Kazuo Ohashi,Shigeki Mukobata et al.
Kohei Tatsumi et al.
Coagulation factor IX (FIX) is synthesized by hepatocytes, and the lack of this protein causes hemophilia B. Liver nonparenchymal cells, including liver sinusoidal endothelial cells (LSECs) and extrahepatic cells in the body, are scarcely s...