The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient [0.03%]
大容量瓶创建连续密度梯度以评估胰岛纯化方法
Chika Miyagi-Shiohira,Naoya Kobayashi,Issei Saitoh et al.
Chika Miyagi-Shiohira et al.
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this m...
Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture [0.03%]
三维培养法体外微环境构建促进的人脂肪干细胞增殖及成脂分化能力的研究：类脂肪组织的形成
Yoshitaka Miyamoto,Masashi Ikeuchi,Hirofumi Noguchi et al.
Yoshitaka Miyamoto et al.
The application of stem cells for cell therapy has been extensively studied in recent years. Among the various types of stem cells, human adipose tissue-derived stem cells (ASCs) can be obtained in large quantities with relatively few passa...
Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice [0.03%]
脂肪来源干细胞对刀豆蛋白A诱导的小鼠急性肝损伤的免疫调节作用
Yasuma Yoshizumi,Hiroshi Yukawa,Ryoji Iwaki et al.
Yasuma Yoshizumi et al.
Cell therapy with adipose tissue-derived stem cells (ASCs) is expected to be a candidate for the treatment of fulminant hepatic failure (FHF), which is caused by excessive immune responses. In order to evaluate the therapeutic effects of AS...
Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells [0.03%]
人脂肪源性间充质干细胞无血清、无异种 cryopreservation溶液的评估
Chika Miyagi-Shiohira,Naoya Kobayashi,Issei Saitoh et al.
Chika Miyagi-Shiohira et al.
Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method ...
Eiji Kobayashi
Eiji Kobayashi
The described research methods explain how you could generate a three-dimensional kidney, based on recent research results. The first method is to fabricate human organs in a pig body. The second is to transplant the so-called "organ bud" i...
Junko Haga,Shin Enosawa,Eiji Kobayashi
Junko Haga
Advances in stem cell research suggest that cell therapy is a potential alternative to liver transplantation. The use of individualized and minimally invasive cell therapy is desirable to avoid rejection and reduce patient burden. While all...
Facilitating Transplantation [0.03%]
促进移植
Hirofumi Noguchi
Hirofumi Noguchi
Side Population Cells From an Immortalized Human Liver Epithelial Cell Line Exhibit Hepatic Stem-Like Cell Properties [0.03%]
永生化的人肝上皮细胞系中的侧群细胞表现出肝干细胞样特性
Takayoshi Tokiwa,Taisuke Yamazaki,Shin Enosawa
Takayoshi Tokiwa
The existence of hepatic stem cells in human livers is controversial. We investigated whether the side population (SP) cells derived from an immortalized human liver epithelial cell line THLE-5b possess the properties of hepatic stem-like c...
Isolation of Hepatic Progenitor Cells From Human Liver With Cirrhosis Secondary to Biliary Atresia Using EpCAM or Thy-1 Markers [0.03%]
应用EpCAM或Thy-1标记物从胆道闭锁继发肝硬化的人肝脏中分离肝前体细胞
Taisuke Yamazaki,Shin Enosawa,Mureo Kasahara et al.
Taisuke Yamazaki et al.
We sought to determine whether hepatic progenitor cells can be isolated from cirrhotic liver using epithelial cell adhesion molecule (EpCAM) or Thy-1 markers. Liver tissue with cirrhosis secondary to biliary atresia (BA) was collagenase dig...
Cell Therapy Using Adipose-Derived Stem Cells for Chronic Liver Injury in Mice [0.03%]
采用脂肪来源干细胞治疗小鼠慢性肝损伤的细胞疗法研究
Kazuo Ohashi,Yoshinori Matsubara,Kohei Tatsumi et al.
Kazuo Ohashi et al.
The present study investigated whether transplantation of autologous adipose-derived stem cells (ASCs) administered into the systemic circulation of a mouse with chronic liver injury provides therapeutic efficacy in the absence of any undes...