Long-term Cryopreservation of Human and other Mammalian Cells at -80 °C for 8 Years [0.03%]
-80℃下八年长期保存人和其它哺乳动物细胞
Yoshitaka Miyamoto,Masashi Ikeuchi,Hirofumi Noguchi et al.
Yoshitaka Miyamoto et al.
Freezing is recognized as the most effective method of maintaining a stable supply of various cell types for long-term storage. However, cells might be damaged by environmental changes during the freezing process. There are various factors ...
Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue [0.03%]
评估用于制备连续密度梯度和装填组织的胰岛纯化方法
Nana Ebi,Chika Miyagi-Shiohira,Eri Hamada et al.
Nana Ebi et al.
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from po...
Therapeutic Cocktail Approach for Treatment of Hyperhomocysteinemia in Alzheimer's Disease [0.03%]
用于阿尔茨海默病同型半胱氨酸血症治疗的联合疗法研究进展
Michael Leon,Darrell Sawmiller,R Douglas Shytle et al.
Michael Leon et al.
In the United States, Alzheimer's disease (AD) is the most common cause of dementia, accompanied by substantial economic and emotional costs. During 2015, more than 15 million family members who provided care to AD patients had an estimated...
Higher Serum Alanine Transaminase Levels in Male Urokinase-Type Plasminogen Activator-Transgenic Mice Are Associated With Improved Engraftment of Hepatocytes but not Liver Sinusoidal Endothelial Cells [0.03%]
较高血清丙氨酸氨基转移酶水平的男性尿激酶型纤溶酶原激活剂转基因小鼠与更好的肝细胞植入有关，但与肝窦状隙内皮细胞无关
Marina E Fomin,Ashley I Beyer,Jean Publicover et al.
Marina E Fomin et al.
The effects of sex on the degree of liver damage and human cell engraftment were investigated in immunodeficient urokinase-type plasminogen activator-transgenic (uPA-NOG) mice. Liver damage, measured by serum alanine transaminase (ALT) leve...
Human Adipose-Derived Mesenchymal Stem Cells Respond to Short-Term Hypoxia by Secreting Factors Beneficial for Human Islets In Vitro and Potentiate Antidiabetic Effect In Vivo [0.03%]
人脐带间充质干细胞分泌的蛋白质因子或可改善胰岛功能和促进胰岛增殖
Simen W Schive,Mohammad Reza Mirlashari,Grete Hasvold et al.
Simen W Schive et al.
Adipose-derived mesenchymal stem cells (ASCs) release factors beneficial for islets in vitro and protect against hyperglycemia in rodent models of diabetes. Oxygen tension has been shown to induce metabolic changes and alter ASCs' release o...
A Prospective, Nonrandomized, no Placebo-Controlled, Phase I/II Clinical Trial Assessing the Safety and Efficacy of Intramuscular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Patients With Severe Buerger's Disease [0.03%]
贝氏病严重患者自体脂肪组织来源间充质干细胞肌肉注射治疗的I/II期临床试验（非随机，未设安慰剂）的安全性和有效性：前瞻性研究
Jeong Chan Ra,Euicheol C Jeong,Sung Keun Kang et al.
Jeong Chan Ra et al.
Buerger's disease is a rare and severe disease affecting the blood vessels of the limbs. Adipose tissue-derived mesenchymal stem cells (ADSCs) have the potential to cure Buerger's disease when developed as a stem cell drug. In the present s...
Neurovascular Cell Sheet Transplantation in a Canine Model of Intracranial Hemorrhage [0.03%]
颅内出血犬模型的神经血管细胞片移植
Woo-Jin Lee,Jong Young Lee,Keun-Hwa Jung et al.
Woo-Jin Lee et al.
Cell-based therapy for intracerebral hemorrhage (ICH) has a great therapeutic potential. However, methods to effectively induce direct regeneration of the damaged neural tissue after cell transplantation have not been established, which, if...
Regenerative Rehabilitation: An Innovative and Multifactorial Approach to Recovery From Stroke and Brain Injury [0.03%]
再生康复：卒中和脑损伤恢复的创新多因素方法
Samantha M Portis,Paul R Sanberg
Samantha M Portis
There is currently a dearth of treatment options for stroke or traumatic brain injury that can restore cognitive and motor function. Regenerative and translational medicine have ushered forth promising new methods for mediating recovery in ...
Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching [0.03%]
通过微流控分子拉伸测量CpG高甲基化引起的DNA长度变化
Daisuke Onoshima,Naoko Kawakita,Daiki Takeshita et al.
Daisuke Onoshima et al.
Abnormal DNA methylation in CpG-rich promoters is recognized as a distinct molecular feature of precursor lesions to cancer. Such unintended methylation can occur during in vitro differentiation of stem cells. It takes place in a subset of ...
Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification [0.03%]
不同渗透压的净化溶液对猪胰岛分离的影响比较研究
Chika Miyagi-Shiohira,Naoya Kobayashi,Issei Saitoh et al.
Chika Miyagi-Shiohira et al.
The osmolality of the purification solution is one of the most critical variables in human islet purification during islet isolation. We previously reported the effectiveness of a combined continuous density/osmolality gradient for the supp...