Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil [0.03%]
来源于巴西东北沿海平原的木质霉菌IS-05的纤维素酶的产生及部分性质分析
Jackeline Pereira Andrade,Aline Simões da Rocha Bispo,Phellippe Arthur Santos Marbach et al.
Jackeline Pereira Andrade et al.
This study evaluated the production of cellulolytic enzymes by Trichoderma sp. IS-05 strain, isolated from sand dunes, according to its ability to grow on cellulose as carbon source. Wheat bran was tested as the carbon source and peptone te...
An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD-Dependent Glutamate Dehydrogenase [0.03%]
对调控芽孢杆菌NAD依赖性谷氨酸脱氢酶辅酶特异性的关键残基进行定点突变分析
Joanna Griffin,Paul C Engel
Joanna Griffin
Sequence and structure comparisons of various glutamate dehydrogenases (GDH) and other nicotinamide nucleotide-dependent dehydrogenases have potentially implicated certain residues in coenzyme binding and discrimination. We have mutated key...
Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase [0.03%]
乳酸菌LBL-4氨肽酶脱苦蛋白水解物中的苦味肽
Bozhidar Tchorbanov,Margarita Marinova,Lydia Grozeva
Bozhidar Tchorbanov
Yoghurt strain Lactobacillus LBL-4 cultivated for 8-10 h at pH ~6.0 was investigated as a considerable food-grade source of intracellular aminopeptidase. Cell-free extract manifesting >200 AP U/l was obtained from cells harvested from 1 L c...
Daniel J Loegering,Michelle R Lennartz
Daniel J Loegering
Protein kinase C (PKC) is a family of kinases that are implicated in a plethora of diseases, including cancer and cardiovascular disease. PKC isoforms can have different, and sometimes opposing, effects in these disease states. Toll-like re...
Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase [0.03%]
鬼笔环肽标记的UDP-半乳糖吡喃糖异构酶荧光偏振结合测定法
Jun Qi,Michelle Oppenheimer,Pablo Sobrado
Jun Qi
Aspergillus fumigatus is an opportunistic human pathogenic fungus responsible for deadly lung infections in immunocompromised individuals. Galactofuranose (Galf) residues are essential components of the cell wall and play an important role ...
Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis [0.03%]
用于生物柴油合成的铌酸盐固定化脂肪酶的形态学、生化和动力学性质评估
Michele Miranda,Daniele Urioste,Livia T Andrade Souza et al.
Michele Miranda et al.
Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL ...
Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor [0.03%]
固定化白腐真菌Trametes versicolor漆酶催化雌二醇氧化反应
Chantale Cardinal-Watkins,Jim A Nicell
Chantale Cardinal-Watkins
Many natural and synthetic estrogens are amenable to oxidation through the catalytic action of oxidative enzymes such as the fungal laccase Trametes versicolor. This study focused on characterizing the conversion of estradiol (E(2)) using l...
Relative Expression Levels Rather Than Specific Activity Plays the Major Role in Determining In Vivo AKT Isoform Substrate Specificity [0.03%]
相对表达量而不是特异活性在体内起主要作用决定AKT同工型底物的特异性
Rachel S Lee,Colin M House,Briony E Cristiano et al.
Rachel S Lee et al.
The AKT protooncogene mediates many cellular processes involved in normal development and disease states such as cancer. The three structurally similar isoforms: AKT1, AKT2, and AKT3 exhibit both functional redundancy and isoform-specific f...
Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System: Optimization through Response Surface Methodology [0.03%]
响应面法优化漆酶介体体系脱色溶苯基红3BL聚氮杂苯染料
Mohamed Neifar,Atef Jaouani,Amel Kamoun et al.
Mohamed Neifar et al.
The decolorization of direct Solophenyl red 3BL (SR), a polyazo dye extensively used in textile industry was studied. The Fomes fomentarius laccase alone did not decolorize SR. The natural redox mediator, acetosyringone (AS), was necessary ...
Amylolytic microorganism from são paulo zoo composting: isolation, identification, and amylase production [0.03%]
来自圣保罗动物园堆肥的淀粉分解微生物：分离、鉴定及产氨酶性能
Renata C Pascon,Rogério Faria Bergamo,Rafael Xavier Spinelli et al.
Renata C Pascon et al.
Composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. This transformation is the result of the microorganism action, converting complex carbon ...