Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus [0.03%]
Pyrococcus furiosus 4-脱氢喹酸合成酶的表达、纯化及性质分析
Leonardo Negron,Mark L Patchett,Emily J Parker
Leonardo Negron
Dehydroquinate synthase (DHQS) catalyses the second step of the shikimate pathway to aromatic compounds. DHQS from the archaeal hyperthermophile Pyrococcus furiosus was insoluble when expressed in Escherichia coli but was partially solubili...
Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes [0.03%]
利用油脂制油饼进行半固态发酵生产低温活性细菌脂肪酶
Babu Joseph,Supriya Upadhyaya,Pramod Ramteke
Babu Joseph
Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus rose...
Enolase: a key player in the metabolism and a probable virulence factor of trypanosomatid parasites-perspectives for its use as a therapeutic target [0.03%]
丙二酸酶：寄生虫代谢和潜在毒力因子的重要参与者-有望成为治疗靶点的新颖药物分子的作用机制研究展望
Luisana Avilán,Melisa Gualdrón-López,Wilfredo Quiñones et al.
Luisana Avilán et al.
Glycolysis and glyconeogenesis play crucial roles in the ATP supply and synthesis of glycoconjugates, important for the viability and virulence, respectively, of the human-pathogenic stages of Trypanosoma brucei, Trypanosoma cruzi, and Leis...
Enzymatic Synthesis of Isopropyl Acetate by Immobilized Bacillus cereus Lipase in Organic Medium [0.03%]
固定化枯草芽孢杆菌脂酶有机介质中合成异丙酸醋
Madan Lal Verma,Wamik Azmi,Shamsher Singh Kanwar
Madan Lal Verma
Selective production of fragrance fatty acid ester from isopropanol and acetic acid has been achieved using silica-immobilized lipase of Bacillus cereus MTCC 8372. A purified thermoalkalophilic extracellular lipase was immobilized by adsorp...
Raffaele Porta,Ashok Pandey,Cristina M Rosell
Raffaele Porta
Screen-printed carbon electrodes modified by rhodium dioxide and glucose dehydrogenase [0.03%]
以二氧化铑和葡萄糖脱氢酶修饰的丝网印刷碳电极
Vojtěch Polan,Jan Soukup,Karel Vytřas
Vojtěch Polan
The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on ...
Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae [0.03%]
酿酒酵母中来自黄色荚膜组织胞浆菌的芽孢杆菌外切酶Amy1的生化和结构特征分析
Alexsandro Sobreira Galdino,Roberto Nascimento Silva,Muriele Taborda Lottermann et al.
Alexsandro Sobreira Galdino et al.
An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme was activ...
Subbarao V Madhunapantula,Gavin P Robertson
Subbarao V Madhunapantula
Identification of key enzymes regulating melanoma progression and drug resistance has the potential to lead to the development of novel, more effective targeted agents for inhibiting this deadly form of skin cancer. The Akt3, also known as ...
Masakazu Kawaguchi,Vincent J Hearing
Masakazu Kawaguchi
A disintegrin and metalloproteinases (ADAMs) are members of a new gene family of transmembrane and secreted proteins, which belong to the zinc proteinase superfamily. These molecules are involved in various biological events such as cell ad...
Neena Philips,Susan Auler,Raul Hugo et al.
Neena Philips et al.
Matrix metalloproteinases (MMPs) are essential to the remodeling of the extracellular matrix. While their upregulation facilitates aging and cancer, they are essential to epidermal differentiation and the prevention of wound scars. The phar...