Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations [0.03%]
利用分子动力学模拟研究CRISPR-Cas12b的温度稳定性
Yinhao Jia,Katelynn Horvath,Santosh R Rananaware et al.
Yinhao Jia et al.
The thermal stability of CRISPR-Cas nucleases is a critical factor for their successful application in 'one-pot' diagnostic assays that utilize high-temperature isothermal amplification. To understand the atomistic mechanism of stabilizatio...
Engineering an indoleamine 2,3-dioxygenase immunotherapy via selective cysteine-to-serine mutations [0.03%]
通过选择性半胱氨酸到丝氨酸的突变工程化诱导酶2,3-氧还蛋白免疫疗法
Jennifer A Simonovich,Arun Wanchoo,Ryan A Clark et al.
Jennifer A Simonovich et al.
Indoleamine 2,3-dioxygenase is an immunomodulatory enzyme that shows great promise when delivered exogenously as a protein therapeutic. However, IDO activity is under complex redox control, mediated in part by multiple cysteine residues wit...
Supramolecular assembly of multifunctional protein gels via an N-glycosylation consensus sequence fusion domain [0.03%]
通过N-糖基化共识序列融合域组装多功能蛋白凝胶的超分子组装体
Eric D Hill,Stephen Michel,Natasha R Sequeira et al.
Eric D Hill et al.
Polypeptide fusion tags that can direct the assembly of folded proteins into supramolecular networks are attractive for creating functional biomaterials. A practical challenge is identifying polypeptide sequences that form supramolecular ne...
Role of antibody heavy and light chain interface residues in affinity maturation of binding to HIV envelope glycoprotein [0.03%]
抗体的高可变性互补决定区如何利用重链和轻链结合界面的氨基酸进行成熟并提高与HIV包膜糖蛋白结合亲和力
Alberto Cisneros rd,Rachel Stecker Nargi,Erica Hammaker Parrish et al.
Alberto Cisneros rd et al.
The FV region of an antibody consists of the heavy chain (HC) and light chain (LC) variable domains whose association is maintained by a series of conserved, non-polar interactions. During chronic infections, somatic mutations are induced, ...
Enhanced thermal response of 3D-printed bilayer hydrogels via nanoclay incorporation [0.03%]
通过纳米粘土增强的3D打印双层水凝胶的热响应性能
Francis Klincewicz,Subhash Kalidindi,Siyuan Liu et al.
Francis Klincewicz et al.
There exist numerous opportunities to design stimuli-responsive bilayer hydrogels for enhanced actuation using simple and robust techniques. Specifically, digital light processing (DLP) 3D printing offers a robust technique for multi-layere...
Harnessing peptide-cellulose interactions to tailor the performance of self-assembled, injectable hydrogels [0.03%]
利用肽-纤维素相互作用量身定制自组装可注射水凝胶的性能
Jessica A Thomas,Alex H Balzer,Subhash Kalidindi et al.
Jessica A Thomas et al.
Taking inspiration from natural systems, such as spider silk and mollusk nacre, that employ hierarchical assembly to attain robust material performance, we leveraged matrix-filler interactions within reinforced polymer-peptide hybrids to cr...
Toward understanding biomolecular materials comprising intrinsically disordered proteins via simulation and experiment [0.03%]
通过模拟和实验理解由固有无序蛋白组成的生物分子材料
Bin Wang,Tianren Zhang,Sirui Shen et al.
Bin Wang et al.
Intrinsically disordered proteins (IDPs) yield solutions with tunable phase transition behavior and have been widely applied in designing stimuli-responsive materials. Understanding interactions between amino acid residues of the IDP sequen...
Construction of an organic cage-based porous ionic liquid using an aminal tying strategy [0.03%]
基于有机笼的多孔离子液体的合成-联胺策略构筑
Aiting Kai,Austin Mroz,Kim E Jelfs et al.
Aiting Kai et al.
An aminal tying method was applied to post-synthetically modify a flexible organic cage, RCC1, to construct a porous ionic liquid (PIL). The resulting PIL, [RCC1-IM][NTf2]6, displayed melting behaviour below 100 °C, a transition to a glass...
Molecular analysis and design using generative artificial intelligence via multi-agent modeling [0.03%]
基于多智能体模型的生成式人工智能在分子分析与设计中的应用
Isabella Stewart,Markus J Buehler
Isabella Stewart
We report the use of a multiagent generative artificial intelligence framework, the X-LoRA-Gemma large language model (LLM), to analyze, design and test molecular design. The X-LoRA-Gemma model, inspired by biological principles and featuri...
Process-based screening of porous materials for vacuum swing adsorption based on 1D classical density functional theory and PC-SAFT [0.03%]
基于一维经典密度函数理论和PC-SAFT的真空变温吸附过程材料筛选方法研究
Fabian Mayer,Benedikt Buhk,Johannes Schilling et al.
Fabian Mayer et al.
Adsorption-based processes are showing substantial potential for carbon capture. Due to the vast space of potential solid adsorbents and their influence on the process performance, the choice of the material is not trivial but requires syst...