Optimisation of Xenium automated in situ sequencing for PAXgene-fixed tissue samples [0.03%]
用于PAXgene固定组织样本的Xenium自动化原位测序优化
Kenny Roberts,Andrew R Bassett
Kenny Roberts
Spatial transcriptomics has transformed the study of gene expression in tissue samples, yet its application to non-standard sample preservation formats beyond fresh frozen and FFPE remains underexplored. PAXgene fixation offers advantages f...
Unraveling myoepithelial cell plasticity in breast cancer: a transcriptomic approach [0.03%]
从转录组学角度解析乳腺癌中肌上皮细胞的可塑性
Mohamed M Haq,Curtis Benson,Meik Kunz et al.
Mohamed M Haq et al.
Myoepithelial cells (MECs) are integral to mammary gland physiology, classically serving a structural and tumor-suppressive role. While their function in normal breast tissue and ductal carcinoma in situ is well characterized, their behavio...
USP34 modulates mitochondrial function in triple-negative breast cancer cells through the eIf3m/MTCH2 axis [0.03%]
USP34通过eIf3m/MTCH2轴调节三阴性乳腺癌细胞的线粒体功能
Peng-Fei Qian,Yi Zeng,Wang-Jing Zhong
Peng-Fei Qian
Triple-negative breast cancer (TNBC) represents a particularly aggressive form of breast tumors. Mitochondrial dysfunction represses the proliferation of TNBC cells. Ubiquitin-specific proteases 34 (USP34) has been predicted to be abnormall...
A look at scanner introduced variation in contrast, resolution, and colour across nine different models of whole slide imaging (WSI) scanner [0.03%]
关于九种全切片影像扫描仪(wsi scanner)的对比度、分辨率和色彩偏差的研究
Hayley Pye,David S Brettle,Danny Kaye et al.
Hayley Pye et al.
Variation in digital whole slide images can be introduced across entire the digital pathology pathway, but one source of variation is the WSI scanner itself. We measured the range of variation across 9 different makes and models of WSI scan...
TissuStamp: a novel high-throughput tissue transfer workflow for FFPE spatial biology assays [0.03%]
一种新型的高通量组织转移工作流程用于福尔马林固定石蜡包埋的空间生物学检测(TissuStamp)
Albert Velasco Abadia,Gina Benedetto,Zhenmin Hong et al.
Albert Velasco Abadia et al.
Spatial biology is shifting from single-sample studies to cohort-scale analyses required for translational research, AI model development, and clinical deployment, yet tissue preparation methods have remained largely unchanged for decades, ...
Application of an open-source AI tool for quantitative quality control in whole slide images of prostate needle core biopsies - a retrospective study [0.03%]
一种开源人工智能工具在前列腺针芯活检全片图像定量质量控制中的应用——一项回顾性研究
Gonçalo Borrecho,Luís Rato,Pedro Salgueiro et al.
Gonçalo Borrecho et al.
The quality of Whole Slide Images (WSI) is a determining factor for proper diagnosis and prognosis, and for enhanced performance of Digital and Computational Pathology. In a context where diagnoses are increasingly quantitative, an automate...
An optimized method for monitoring blood-brain barrier penetration of small-molecule fluorescent probes [0.03%]
一种优化的小分子荧光探针血脑屏障通透性监测方法
Wenyi He,Xue Yu,Yinuo Li et al.
Wenyi He et al.
Accurate assessment of blood-brain barrier (BBB) penetration is essential for validating small-molecule fluorescent probes. Conventional methods often suffer from signal attenuation, tissue damage, or fluorescence distortion during chemical...
Color standardization in whole slide imaging: a method to reduce color variability [0.03%]
全片扫描图像中的颜色标准化：减少颜色变化的方法
Louise Collins,Richard Heale,Richard M Salmon
Louise Collins
Color standardization in digital pathology is essential for ensuring consistency across Whole Slide Imaging (WSI) scanners. This study utilizes color data from multiple WSI scanners captured from a slide containing 55 color patches with his...
Beyond algorithmic performance: translational gaps in implementing artificial intelligence for clinical digital pathology [0.03%]
超越算法性能：临床数字病理学人工智能实施中的转化差距
Rong Xia,Nickolas Littlefield,Riyue Bao et al.
Rong Xia et al.
Morphometric analysis and its application in lupus nephritis: a systematic review [0.03%]
形态学分析及其在狼疮性肾炎中的应用：系统综述
Lucia Mercedes Niño-Hernandez,Liria Terradez Mas,Amparo Ruiz Sauri
Lucia Mercedes Niño-Hernandez
Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus (SLE), characterized by marked histological heterogeneity and high interobserver variability in the evaluation of renal biopsies. Digital morphometry has emerged...