The evolving role of the histotechnologist in digital, value based healthcare [0.03%]
数字化、基于价值的医疗模式下细胞病理技师的角色变迁
Ankush U Patel,Danielle Pirain,Monika Lamba Saini
Ankush U Patel
Digital pathology and value-based healthcare (VBHC) are converging to reshape diagnostic medicine, yet the histotechnologist, whose tissue preparation enables every glass slide and whole slide image (WSI), remains largely invisible in this ...
An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections [0.03%]
大面积组织切片的DNA荧光原位杂交和蛋白质检测的综合方案
Eleanor M O Roberts,Pranauti Panshikar,Xiaoze Li Wang et al.
Eleanor M O Roberts et al.
Different omics types such as genomics and proteomics all contribute to deciphering biology. Applying these omics approaches in a spatial context helps reveal biology in situ at a single cell level. Here we present a protocol for the combin...
The impact of stain variation and colour normalisation on tissue stain intensity and automated nuclear counting: a multi-site study [0.03%]
染色差异及颜色归一化对组织切片染色强度和自动核计数的影响：一项多中心研究
Catriona Dunn,David Brettle,Peter Ellery et al.
Catriona Dunn et al.
Variation in H&E staining is often a confounder when comparing data in multi-site studies, and impacts on the generalisability of AI models. In this paper, slides containing serial sections of control liver tissue were sent out to eight cli...
Optimisation of Xenium automated in situ sequencing for PAXgene-fixed tissue samples [0.03%]
用于PAXgene固定组织样本的Xenium自动化原位测序优化
Kenny Roberts,Andrew R Bassett
Kenny Roberts
Spatial transcriptomics has transformed the study of gene expression in tissue samples, yet its application to non-standard sample preservation formats beyond fresh frozen and FFPE remains underexplored. PAXgene fixation offers advantages f...
Unraveling myoepithelial cell plasticity in breast cancer: a transcriptomic approach [0.03%]
从转录组学角度解析乳腺癌中肌上皮细胞的可塑性
Mohamed M Haq,Curtis Benson,Meik Kunz et al.
Mohamed M Haq et al.
Myoepithelial cells (MECs) are integral to mammary gland physiology, classically serving a structural and tumor-suppressive role. While their function in normal breast tissue and ductal carcinoma in situ is well characterized, their behavio...
USP34 modulates mitochondrial function in triple-negative breast cancer cells through the eIf3m/MTCH2 axis [0.03%]
USP34通过eIf3m/MTCH2轴调节三阴性乳腺癌细胞的线粒体功能
Peng-Fei Qian,Yi Zeng,Wang-Jing Zhong
Peng-Fei Qian
Triple-negative breast cancer (TNBC) represents a particularly aggressive form of breast tumors. Mitochondrial dysfunction represses the proliferation of TNBC cells. Ubiquitin-specific proteases 34 (USP34) has been predicted to be abnormall...
A look at scanner introduced variation in contrast, resolution, and colour across nine different models of whole slide imaging (WSI) scanner [0.03%]
关于九种全切片影像扫描仪(wsi scanner)的对比度、分辨率和色彩偏差的研究
Hayley Pye,David S Brettle,Danny Kaye et al.
Hayley Pye et al.
Variation in digital whole slide images can be introduced across entire the digital pathology pathway, but one source of variation is the WSI scanner itself. We measured the range of variation across 9 different makes and models of WSI scan...
TissuStamp: a novel high-throughput tissue transfer workflow for FFPE spatial biology assays [0.03%]
一种新型的高通量组织转移工作流程用于福尔马林固定石蜡包埋的空间生物学检测(TissuStamp)
Albert Velasco Abadia,Gina Benedetto,Zhenmin Hong et al.
Albert Velasco Abadia et al.
Spatial biology is shifting from single-sample studies to cohort-scale analyses required for translational research, AI model development, and clinical deployment, yet tissue preparation methods have remained largely unchanged for decades, ...
Application of an open-source AI tool for quantitative quality control in whole slide images of prostate needle core biopsies - a retrospective study [0.03%]
一种开源人工智能工具在前列腺针芯活检全片图像定量质量控制中的应用——一项回顾性研究
Gonçalo Borrecho,Luís Rato,Pedro Salgueiro et al.
Gonçalo Borrecho et al.
The quality of Whole Slide Images (WSI) is a determining factor for proper diagnosis and prognosis, and for enhanced performance of Digital and Computational Pathology. In a context where diagnoses are increasingly quantitative, an automate...
An optimized method for monitoring blood-brain barrier penetration of small-molecule fluorescent probes [0.03%]
一种优化的小分子荧光探针血脑屏障通透性监测方法
Wenyi He,Xue Yu,Yinuo Li et al.
Wenyi He et al.
Accurate assessment of blood-brain barrier (BBB) penetration is essential for validating small-molecule fluorescent probes. Conventional methods often suffer from signal attenuation, tissue damage, or fluorescence distortion during chemical...