High Expression of Methylotrophic Yeast-Derived Recombinant Human Erythropoietin in a pH-Controlled Batch System [0.03%]
采用pH控制培养基对甲酸盐呼吸型毕赤酵母P.pastoris生产高比活性人红细胞生成素(EPO)蛋白质的研究
Ahmad Maleki,Farzin Roohvand,Hosnieh Tajerzadeh et al.
Ahmad Maleki et al.
To accomplish the worldwide demand for recombinant human erythropoietin (rHuEpo) as a therapeutic, application of cost-efficient expression system of methylotrophic yeast Pichia pastoris (P. pastoris) rather than mammalian cells is indispen...
Producing a Mammalian GFP Expression Vector Containing Neomycin Resistance Gene [0.03%]
含有新霉素抗性基因的哺乳动物GFP表达载体的构建
Manizheh Izadi,Maryam Abiri,Mohammad Keramatipour
Manizheh Izadi
The green fluorescent protein (GFP) was originally isolated from the Jellyfish Aequorea Victoria that fluoresces green when exposed to blue light. GFP protein is composed of 238 amino acids with the molecular mass of 26.9 kD. The GFP gene i...
Recombinase Polymerase Amplification (RPA)-ELISA as an Isothermal Molecular POCT Method for Bacterial Respiratory Infection Diagnosis [0.03%]
用于细菌性呼吸道感染诊断的重组酶聚合酶扩增（RPA）-ELISA等温分子POCT方法
Background: Acute Respiratory Infections (ARIs) are a leading cause of childhood mortality worldwide, especially in African and Southeast Asian countries. Point of Care Test (POCT) techniques provide faster diagnoses comp...