Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds [0.03%]
兰科药用珍稀植物兜唇石仙桃成熟种子的异养萌发和叶片再生体系建立
Yage Tu,Jianming Wang,Zhechen Qi et al.
Yage Tu et al.
Hemipilia cucullata (Orchidaceae) is a terrestrial orchid widely valued for its medicinal properties and ornamental appeal, yet its wild populations are endangered due to habitat degradation, low seed viability, and limited pollination succ...
Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting [0.03%]
优化的蛙卵和胚胎蛋白质免疫印迹分析方法
Charlotte R Kanzler,Michael D Sheets
Charlotte R Kanzler
Analysis of proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting (western blotting) is a vital part of the molecular biologist's toolkit. This technique separates a complex protein mix...
FRET Dilution Assay for Analyzing Dynamic Exchange Between Protein Assemblies [0.03%]
用于分析蛋白质聚集体之间动态交换的FRET稀释测定法
Reid Gordon,Daniel E Morse
Reid Gordon
As the molecular driver for tunable iridescence in cephalopods, the tunable phase behavior of reflectin A1 protein continues to be a focus of biomaterial engineering. Modulating salt concentration and protein net charge density of reflectin...
Diandian Jiao,Ping Zhou
Diandian Jiao
Premature ovarian insufficiency (POI) is a critical condition leading to female infertility, necessitating reliable animal models for mechanistic and therapeutic research. Here, we present a standardized protocol for establishing and evalua...
Evaluating the Effect of SASP Factors on the Proliferation of Cancer Cells using A Comparative Analysis of Three Distinct Methodologies [0.03%]
三种不同方法评估SASP因素对癌细胞增殖影响的比较分析研究
Yaprak Dilber Şimay Demir,Tuğçe Tayyar,Aysun Özdemir et al.
Yaprak Dilber Şimay Demir et al.
Chemotherapy-induced senescent cancer cells also secrete various factors, called senescence-associated secretory phenotype, to regulate their extracellular microenvironment. Previous studies have reported that these SASP factors exert detri...
Comparative Study
Journal of visualized experiments : JoVE. 2025 Sep 19:(223). DOI:10.3791/68883 2025
Yunxia Wang,Chunling Xue,Qiuying Luo et al.
Yunxia Wang et al.
Mycoplasma pneumoniae (Mp) represents one of the most prevalent pathogens responsible for community-acquired pneumonia (CAP) in pediatric populations. The detection of Mycoplasma pneumoniae resistance genes provides robust technical support...
Impact of Subcutaneous Mastectomy under Laparoscopy with In Situ Prosthetic Reconstruction on Aesthetic Appearance in Breast Cancer Patients [0.03%]
腹腔镜下乳腺癌保留皮肤麦默通微创切除术的美容效果分析
Guojian Shi,Lili Hou,Lei Yin et al.
Guojian Shi et al.
Breast cancer remains one of the most widespread malignant tumors affecting women, with a range of treatment options available, among which surgical intervention holds a central position. Traditional mastectomy, while effective in removing ...
Modeling Dysplastic and Functional Lung Alveolar Repair after Influenza Infection [0.03%]
流感感染后肺泡异常增生和功能修复的建模研究
Sachin Gaurav,Arnav Sharma,Francisco Lobo et al.
Sachin Gaurav et al.
Viral infection causes both acute and long-term damage to the lung alveolus, the specialized tissue structure responsible for gas exchange between the cardiovascular system and the external environment. Influenza A virus (IAV) infection in ...
Yongcong Yan,Haohan Liu,Zhiyu Xiao et al.
Yongcong Yan et al.
According to extensive case analyses compiled by the Japanese Study Group on Pancreaticobiliary Maljunction (JSPBM), patients with Pancreaticobiliary Maljunction (PBM) exhibit a significantly elevated incidence of biliary tract tumors, rang...
Application of Optogenetics Combined with Fiber Photometry Recording in the Regulation of Neural Circuits-visceral Functions by Electroacupuncture [0.03%]
光遗传学结合光纤记录电针调节神经环路-内脏功能的作用机制研究
Yueyue Liu,Yun Liu,Ying Wang et al.
Yueyue Liu et al.
This protocol describes a technique for optogenetic manipulation of specific types of neurons and real-time recording of the effects of electroacupuncture at Zusanli (ST36) on nuclear calcium signals and gastric motility in mice under anest...