An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording [0.03%]
一种用于多区域在体记录的灵活便捷电生理-optrode集成制备方法
Yan Tao,Yuxin Zhao,Wenqi Zhong et al.
Yan Tao et al.
Epilepsy is a neurological disorder characterized by synchronized abnormal discharges involving multiple brain regions. Focal lesions facilitate the propagation of epileptic signals through associated neural circuits. Therefore, in vivo rec...
Periorbital Placement of a Laser Doppler Probe for Cerebral Blood Flow Monitoring Prior to Middle Cerebral Artery Occlusion in Rodent Models [0.03%]
激光多普勒探头眶周放置法在啮齿类脑中动脉闭塞模型术前脑血流监测中的应用
Denali C Dickson,Mitchell J Bartlet,Sharon Hom et al.
Denali C Dickson et al.
Middle cerebral artery occlusion (MCAO) is the gold-standard method for preclinical modeling of ischemic stroke in rodents. However, successful occlusion is not guaranteed by even the most skilled surgical hands. Errors primarily occur when...
Hyoin Lee,Dahm So,Jiye Choi et al.
Hyoin Lee et al.
Empathy, characterized by the ability to recognize and share the emotions of others, plays a fundamental role in shaping social interactions. It allows individuals to respond to the emotional states of others, promoting prosocial behaviors ...
Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices [0.03%]
优化和简化精密切割肝切片的制备和培养技术
Dany Perocheau,Claire Duff,Sonam Gurung et al.
Dany Perocheau et al.
This protocol presents a simple system for the creation and culture of Precision-cut Liver Slices (PCLS). PCLS contains all cells in an intact environment and, therefore, resembles a mini model of the whole organ. They enable the study of l...
Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture [0.03%]
大鼠肝灌注和原代肝细胞分离：一个经典的 procedure 在前沿的 3D 器官样培养中的关键作用
Valentina Tiriticco,Gabriele Codotto,Benedetta Blarasin et al.
Valentina Tiriticco et al.
Primary hepatocytes are a commonly used tool for in vitro liver-related studies. However, the maintenance of these cells has always been a challenge due to the rapid loss of morphology, viability, and functionality in culture. A recent appr...
Marta Brambilla,Alessia Becchetti,Kevin Nallio et al.
Marta Brambilla et al.
A subset of circulating human platelets stores Tissue Factor (TF) intracellularly, the key activator of the blood coagulation cascade and thrombus formation. Upon platelet activation, TF is exposed on the cell membrane, where it binds to FV...
Innovative Approach to Minimize Serious Complications in Cosmetic Dermal Filling [0.03%]
创新性微创技术在注射美容中的应用及并发症的防治研讨班
Pengfei Li,Hui Lu
Pengfei Li
Cosmetic dermal filling represents a significant advance in aesthetic medicine, providing non-invasive treatments with immediate and durable outcomes. Despite the generally safe nature of dermal fillers and avoidable complications with prop...
Jie Chen,Luo Yang,Na Yue et al.
Jie Chen et al.
As the global economy grows and living standards improve, the rise in overweight and obese individuals poses a significant public health challenge. This trend is predominantly linked to lifestyle changes and poor dietary habits, resulting i...
Combined Supine and Standing Imaging for Varicocele: An Improved Diagnostic Approach [0.03%]
用于精索静脉曲张的联合仰卧和站立影像诊断方法
Zhiming Xu,Jianying Li,Shimei Li et al.
Zhiming Xu et al.
Varicocele, one of the main diseases affecting male reproductive health, relies heavily on angiographic diagnostic techniques for disease-related examination and assessment. Traditional varicocele angiography often employs a supine position...
Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer [0.03%]
采用细胞外流式分析仪评估谷氨酰胺作为慢性乙醇暴露肺泡巨噬细胞的燃料来源
Kathryn M Crotty,Samantha M Yeligar
Kathryn M Crotty
Alveolar macrophages (AMs) are the first line of cellular defense in the lower airway against pathogens. However, chronic and excessive alcohol use impairs the ability of AMs to phagocytize and clear pathogens from the alveolar space, in pa...