Sizing Up Protein-Ligand Complexes: The Rise of Structural Mass Spectrometry Approaches in the Pharmaceutical Sciences [0.03%]
结构质量测定在药物科学中的应用及结构性质作用复合物的分析方法的发展
Joseph D Eschweiler,Richard Kerr,Jessica Rabuck-Gibbons et al.
Joseph D Eschweiler et al.
Capturing the dynamic interplay between proteins and their myriad interaction partners is critically important for advancing our understanding of almost every biochemical process and human disease. The importance of this general area has sp...
Limor Cohen,David R Walt
Limor Cohen
The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement of analytes of interest into ultrasmall containers have greatly enhanced the sensi...
Angela R Wu,Jianbin Wang,Aaron M Streets et al.
Angela R Wu et al.
Despite being a relatively recent technological development, single-cell transcriptional analysis through high-throughput sequencing has already been used in hundreds of fruitful studies to make exciting new biological discoveries that woul...
Raman Imaging in Cell Membranes, Lipid-Rich Organelles, and Lipid Bilayers [0.03%]
细胞膜、富含脂质的细胞器和脂质双层中的拉曼成像技术
Aleem Syed,Emily A Smith
Aleem Syed
Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use i...
Renee J Tran,Krystal L Sly,John C Conboy
Renee J Tran
Surface second harmonic generation (SHG) is a coherent, nonlinear optical technique that is well suited for investigations of biomolecular interactions at interfaces. SHG is surface specific due to the intrinsic symmetry constraints on the ...
Coupling Front-End Separations, Ion Mobility Spectrometry, and Mass Spectrometry For Enhanced Multidimensional Biological and Environmental Analyses [0.03%]
结合前处理分离、离子淌度和质谱技术以增强多维生物及环境分析能力
Xueyun Zheng,Roza Wojcik,Xing Zhang et al.
Xueyun Zheng et al.
Ion mobility spectrometry (IMS) is a widely used analytical technique for rapid molecular separations in the gas phase. Though IMS alone is useful, its coupling with mass spectrometry (MS) and front-end separations is extremely beneficial f...
Timothy K Toby,Luca Fornelli,Neil L Kelleher
Timothy K Toby
From a molecular perspective, enactors of function in biology are intact proteins that can be variably modified at the genetic, transcriptional, or post-translational level. Over the past 30 years, mass spectrometry (MS) has become a powerf...
Advanced Multidimensional Separations in Mass Spectrometry: Navigating the Big Data Deluge [0.03%]
质谱中的高级多维分离：应对大数据洪流
Jody C May,John A McLean
Jody C May
Hybrid analytical instrumentation constructed around mass spectrometry (MS) is becoming the preferred technique for addressing many grand challenges in science and medicine. From the omics sciences to drug discovery and synthetic biology, m...
Recent Progress in Monolithic Silica Columns for High-Speed and High-Selectivity Separations [0.03%]
高速高选择性分离整体硅胶柱研究新进展
Tohru Ikegami,Nobuo Tanaka
Tohru Ikegami
Monolithic silica columns have greater (through-pore size)/(skeleton size) ratios than particulate columns and fixed support structures in a column for chemical modification, resulting in high-efficiency columns and stationary phases. This ...
Adrian M Schrell,Nikita Mukhitov,Lian Yi et al.
Adrian M Schrell et al.
The release of chemical information from cells and tissues holds the key to understanding cellular behavior and dysfunction. The development of methodologies that can measure cellular secretion in a time-dependent fashion is therefore essen...