Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining [0.03%]
基于BrdU偶联的点击化学细胞增殖法用于多重抗体染色
Paolo Cappella,Fabio Gasparri,Maurizio Pulici et al.
Paolo Cappella et al.
Determination of incorporation of the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) into DNA is a widely used method to analyze the cell cycle. However, DNA denaturation is required for BrdU detection with the consequence that most protei...
Uncompensated Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1 and Multilaser Excitation [0.03%]
基于JC-1和多激光激发的线粒体膜电位偏振光未补偿分析法研究
Sara De Biasi,Lara Gibellini,Andrea Cossarizza
Sara De Biasi
The lipophilic cation JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl carbocyanine iodide) has been used for more than 20 years as a specific dye for measuring mitochondrial membrane potential (ΔΨ(m)). In this unit, we rev...
High-Sensitivity Detection of PNH Red Blood Cells, Red Cell Precursors, and White Blood Cells [0.03%]
高灵敏度检测红细胞、红系前体细胞和白细胞PNH阳性单元的技术
D Robert Sutherland,Andrea Illingworth,Michael Keeney et al.
D Robert Sutherland et al.
Flow cytometry is the method of choice to 'diagnose' paroxysmal nocturnal hemoglobinuria (PNH) and has led to improved patient management. Most laboratories have limited experience with PNH testing, and many different flow approaches are us...
Michael A Model
Michael A Model
Cell volume is an important parameter in cell adaptation to anisosmotic stress, in the development of apoptosis and necrosis, and in the pathogenesis of several diseases. This unit describes a method for measuring the volume of adherent cel...
Application of click chemistry conditions for 5-bromo-2'-deoxyuridine determination through Fenton and related reactions [0.03%]
基于芬顿反应的点击化学条件在5-溴脱氧尿嘧啶核苷检测中的应用研究
Paolo Cappella,Maurizio Pulici,Fabio Gasparri
Paolo Cappella
Mixtures of ascorbate and copper used in certain click chemistry experimental conditions act as oxidizing agents, catalyzing the formation of reactive oxygen species through Fenton and related reactions. Hydroxyl radicals act as chemical nu...
Whole blood measurement of histone modifications linked to the epigenetic regulation of gene expression [0.03%]
与基因表达表观遗传调控相关的组蛋白修饰的全血检测
Maria Watson,David Hedley
Maria Watson
Rapid progress is being made to understand the regulatory mechanisms that underlie the epigenetic control of gene expression through histone modification. It is now recognized that this plays a major role in normal development and disease. ...
Quantification of Th1 and Th17 Cells with Intracellular Staining Following PMA/Ionomycin Stimulation [0.03%]
经PMA/离子霉素刺激后的细胞内染色定量检测Th1和Th17细胞数量
Fridrik Karlsson,Mina Hassan-Zahraee
Fridrik Karlsson
Cytokine-producing cells are at the center of the adaptive immune responses, and quantifying these cells is an important aspect to build understanding of the immune response. In particular, Th1 and Th17 cells have been implicated in the pat...
Michael A Davis,Ondřej Pražský,Laura R Sysko
Michael A Davis
Time-lapse imaging is a rich data source offering potential kinetic information of cellular activity and behavior. Tracking and extracting measurements of objects from time-lapse datasets are challenges that result from the complexity and d...
Michael W Adams,Andrew F Loftus,Sarah E Dunn et al.
Michael W Adams et al.
The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate im...
Mark A Naivar,David W Galbraith
Mark A Naivar
A flow cytometer is made up of many different subsystems that work together to measure the optical properties of individual cells within a sample. The data acquisition system (also called the data system) is one of these subsystems, and it ...