Véronique Wuyts,Nancy H C Roosens,Sophie Bertrand et al.
Véronique Wuyts et al.
Characterization of microbial pathogens is necessary for surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation-PCR (MOL-PCR) optimized for characterization of microbial p...
Quantitative Flow Cytometry Measurements in Antibodies Bound per Cell Based on a CD4 Reference [0.03%]
基于CD4参考的抗体结合定量流式细胞测量值测定(每细胞)
Lili Wang,Heba Degheidy,Fatima Abbasi et al.
Lili Wang et al.
Multicolor flow cytometer assays with fluorescently labeled antibodies are routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens eith...
In Vivo Immuno-Spin Trapping: Imaging the Footprints of Oxidative Stress [0.03%]
体内免疫自旋捕获成像技术：氧化应激的活体高时空分辨成像
Nicholas K H Khoo,Nadiezhda Cantu-Medellin,Claudette St Croix et al.
Nicholas K H Khoo et al.
A plethora of disease processes are associated with elevated reactive species formation and allied reactions with biomolecules that alter cell signaling, induce overt damage, and promote dysfunction of tissues. Unfortunately, effective dete...
Microscopic Investigation of Protein Function in C. elegans Using Fluorescent Imaging [0.03%]
利用荧光成像技术在分子水平上研究线虫中的蛋白质功能
Cliff J Luke,Linda P O&#x;Reilly
Cliff J Luke
Caenorhabditis elegans is a powerful model organism for studying human biology and disease due to its surprisingly high genetic homology to Homo sapiens. Its genetic amenability, small size, short generation time, and transparent body make ...
High Throughput-Based Mitochondrial Function Assays by Multi-Parametric Flow Cytometry [0.03%]
高通量线粒体功能检测的多参数流式细胞术分析方法研究
J Paul Robinson,Nianyu Li,Padma Kumar Narayanan
J Paul Robinson
Mitochondrial dysfunction has been increasingly implicated as an important mechanism for chemical-induced toxicity. In the present unit, we describe a multi-parametric flow cytometry assay to assess the effects of drug or chemical-induced m...
Pradeep K Dagur,J Philip McCoy Jr
Pradeep K Dagur
Human peripheral blood is often studied by flow cytometry in both the research and clinical laboratories. The methods for collection, storage, and preparation of peripheral blood will vary depending on the cell lineage to be examined as wel...
Flow Cytometry of Extracellular Vesicles: Potential, Pitfalls, and Prospects [0.03%]
细胞外囊泡流动式细胞仪分析：前景、潜在问题及展望
John P Nolan
John P Nolan
Evidence suggests that extracellular vesicles (EVs) can play roles in physiology and pathology, providing impetus to explore their use as diagnostic and therapeutic targets. However, EVs are also small, heterogeneous, and difficult to measu...
Visualization of Telomere Integrity and Function In Vitro and In Vivo Using Immunofluorescence Techniques [0.03%]
利用免疫荧光技术体外和体内可视化端粒的完整性与功能
Anthony J Cesare,Christopher M Heaphy,Roderick J O&#x;Sullivan
Anthony J Cesare
In cancer cells, telomere length maintenance occurs largely via the direct synthesis of TTAGGG repeats at chromosome ends by telomerase, or less frequently by the recombination-dependent alternative lengthening of telomeres (ALT) pathway. T...
Avery D Posey Jr,Omkar U Kawalekar,Carl H June
Avery D Posey Jr
Using flow cytometry, single-cell measurements of calcium can be made on isolated populations identified by one or more phenotypic characteristics. Most earlier techniques for measuring cellular activation parameters determined the mean val...
Valeriya Gaysinskaya,Alex Bortvin
Valeriya Gaysinskaya
Protocols for purification of murine male germ cells by FACS based on Hoechst 33342 (Ho342) dye staining have been reported and optimized. However, the protocols are often challenging to follow, partly due to difficulties related to sample ...