Elena I Kovalenko,Maria A Streltsova,Leonid M Kanevskiy et al.
Elena I Kovalenko et al.
Our understanding of NK biology is increased dramatically, a product of improved flow-cytometric techniques for analyzing these cells. NK cells undergo significant changes in repertoire during differentiation. A repeating stimulus, such as ...
Jan Vrána,Petr Cápal,Hana Šimková et al.
Jan Vrána et al.
Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner,...
Anja J Gerrits,Andrew L Frelinger rd,Alan D Michelson
Anja J Gerrits
In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. This leukocyte-platelet aggregate formation is initiated primarily through platelet surface expression of P-sel...
Neil J Kelly,Nadine Dandachi,Dmitry A Goncharov et al.
Neil J Kelly et al.
The quantification of tunica media thickness in histological cross sections is a ubiquitous exercise in cardiopulmonary research, yet the methods for quantifying medial wall thickness have never been rigorously examined with modern image an...
William Telford,Karen Tamul,Jolene Bradford
William Telford
Apoptosis is an important mechanism in cell biology, playing a critical regulatory role in virtually every organ system. It has been particularly well characterized in the immune system, with roles ranging from immature immune cell developm...
High-Content Microscopy Analysis of Subcellular Structures: Assay Development and Application to Focal Adhesion Quantification [0.03%]
高内涵显微镜亚细胞结构分析：焦粘连量化测定的测定开发与应用
Torsten Kroll,David Schmidt,Georg Schwanitz et al.
Torsten Kroll et al.
High-content analysis (HCA) converts raw light microscopy images to quantitative data through the automated extraction, multiparametric analysis, and classification of the relevant information content. Combined with automated high-throughpu...
A Quantitative Method for Comparing the Brightness of Antibody-dye Reagents and Estimating Antibodies Bound per Cell [0.03%]
一种比较抗体-染料试剂亮度并估算每个细胞结合的抗体数量的定量方法
Aaron B Kantor,Wayne A Moore,Stephen Meehan et al.
Aaron B Kantor et al.
We present a quantitative method for comparing the brightness of antibody-dye reagents and estimating antibodies bound per cell. The method is based on complementary binding of test and fill reagents to antibody capture microspheres. Severa...
Comparative Study
Current protocols in cytometry. 2016 Jul 1:77:1.30.1-1.30.23. DOI:10.1002/cpcy.6 2016
Measurement of Low-Abundance Intracellular mRNA Using Amplified FISH Staining and Image-Based Flow Cytometry [0.03%]
基于图像的流式细胞术在扩增FISH染色中的应用及低丰度mRNA检测
Andrea L Henning,Jill N Best Sampson,Brian Keith McFarlin
Andrea L Henning
Recent advances in instrument design and reagent development have enabled the rapid progression in available measurement techniques in the field of flow cytometry. In particular, image-based flow cytometry extends the analysis capacity foun...
Alfonso Schmidt,Tiffany Bouchery,Graham Le Gros et al.
Alfonso Schmidt et al.
Traditional jet-in-air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 μm. We discuss the practical considerations of setting up a conventional jet-in-ai...
Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins [0.03%]
单细胞中同时测量信使RNA、细胞表面蛋白和胞内蛋白的方法
Kah Teong Soh,Joseph D Tario Jr,Sean Colligan et al.
Kah Teong Soh et al.
Nucleic acid content can be quantified by flow cytometry through the use of intercalating compounds; however, measuring the presence of specific sequences has hitherto been difficult to achieve by this methodology. The primary obstacle to d...