Jianquan Xu,Hongqiang Ma,Yang Liu
Jianquan Xu
Super-resolution (SR) fluorescence microscopy, a class of optical microscopy techniques at a spatial resolution below the diffraction limit, has revolutionized the way we study biology, as recognized by the Nobel Prize in Chemistry in 2014....
Method for DNA Ploidy Analysis Along with Immunophenotyping for Rare Populations in a Sample using FxCycle Violet [0.03%]
一种使用FxCycle Violet进行DNA倍体分析并同时对样本中罕见亚群进行免疫分型的方法
Prashant Tembhare,Yajamanam Badrinath,Sitaram Ghogale et al.
Prashant Tembhare et al.
The clinical use of flow cytometric DNA ploidy assay has been extended towards stratifying the risk of diseases, such as monoclonal gammopathies or B cell acute lymphoblastic leukemia, and to detect circulating tumor cells, both of which re...
Method to Detect the Cellular Source of Over-Activated NADPH Oxidases Using NAD(P)H Fluorescence Lifetime Imaging [0.03%]
利用NAD(P)H荧光寿命成像检测活细胞中过激活的NADPH氧化酶的方法
Daniel Bremer,Ruth Leben,Ronja Mothes et al.
Daniel Bremer et al.
Fluorescence-lifetime imaging microscopy (FLIM) is a technique to generate images, in which the contrast is obtained by the excited-state lifetime of fluorescent molecules instead of their intensity and emission spectrum. The ubiquitous coe...
Teresa S Hawley,Robert G Hawley,William G Telford
Teresa S Hawley
Fluorescent proteins have become standard tools for cell and molecular biologists. The color palette of fluorescent proteins spans the ultraviolet, visible, and near-infrared spectrum. Utility of fluorescent proteins has been greatly facili...
Correlative Fluorescence and Electron Microscopy in 3D-Scanning Electron Microscope Perspective [0.03%]
三维扫描电子镜下的相关荧光与电子显微技术
Jonathan Franks,Callen T Wallace,Masateru Shibata et al.
Jonathan Franks et al.
The ability to correlate fluorescence microscopy (FM) and electron microscopy (EM) data obtained on biological (cell and tissue) specimens is essential to bridge the resolution gap between the data obtained by these different imaging techni...
Asya Smirnov,Michael D Solga,Joanne Lannigan et al.
Asya Smirnov et al.
Quantifying the efficiency of particle uptake by host cells is important in the fields of infectious diseases, autoimmunity, cancer, developmental biology, and drug delivery. Here we present a protocol for high-throughput analysis of partic...
Lili Wang,Robert A Hoffman
Lili Wang
Because flow cytometers are designed to measure particle characteristics, particles are the most common materials used to calibrate, control, and standardize the instruments. Definitions and cautions are provided for common terms to alert t...
Measurement of T-Cell Telomere Length Using Amplified-Signal FISH Staining and Flow Cytometry [0.03%]
采用信号扩增FISH染色和流式细胞术测定T细胞端粒长度
Andrea L Henning,Danielle E Levitt,Jakob L Vingren et al.
Andrea L Henning et al.
Exposure to pathogen-associated molecular patterns (PAMPS), damage-associated molecular patterns (DAMPS), and physiologically challenging stimuli either positively or negatively affect leukocyte maturity. Cellular maturity has implications ...
Jing Zhao,Heike Fuhrmann-Stroissnigg,Aditi U Gurkar et al.
Jing Zhao et al.
Cellular senescence refers to the irreversible growth arrest of normally dividing cells in response to various types of stress. Cellular senescence is induced by telomere shortening due to repeated cell division, which causes a DNA damage r...
Theodoros Kelesidis,Ingrid Schmid
Theodoros Kelesidis
Telomere sequences at the end of chromosomes control somatic cell division; therefore, telomere length in a given cell population provides information about its replication potential. This unit describes a method for flow cytometric measure...