Basics of Digital Microscopy [0.03%]
数字显微镜基础
Callen T Wallace,Morgan Jessup,Tytus Bernas et al.
Callen T Wallace et al.
Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer fil...
Kenneth W Dunn,Timothy A Sutton,Ruben M Sandoval
Kenneth W Dunn
Intravital microscopy, microscopy of living animals, is a powerful research technique that combines the resolution and sensitivity found in microscopic studies of cultured cells with the relevance and systemic influences of cells in the con...
Zbigniew Darzynkiewicz,Xuan Huang,Hong Zhao
Zbigniew Darzynkiewicz
Cellular DNA content can be measured by flow cytometry with the aim of : (1) revealing cell distribution within the major phases of the cell cycle, (2) estimating frequency of apoptotic cells with fractional DNA content, and/or (3) disclosi...
Click Chemistry for Analysis of Cell Proliferation in Flow Cytometry [0.03%]
用于流动分析细胞增殖的点击化学方法学研究报告cychrome-flow(TM)
Scott T Clarke,Veronica Calderon,Jolene A Bradford
Scott T Clarke
The measurement of cellular proliferation is fundamental to the assessment of cellular health, genotoxicity, and the evaluation of drug efficacy. Labeling, detection, and quantification of cells in the synthesis phase of cell cycle progress...
Staining of Frozen and Formalin-Fixed, Paraffin-Embedded Tissues with Metal-Labeled Antibodies for Imaging Mass Cytometry Analysis [0.03%]
用于成像质谱细胞分析的金属标记抗体对冷冻和甲醛固定石蜡包埋组织的染色
Qing Chang,Olga Ornatsky,David Hedley
Qing Chang
This unit describes protocols for labeling tissue sections using combinations of metal-tagged antibodies and an iridium-containing DNA intercalator for analysis by imaging mass cytometry. Imaging mass cytometry (IMC) allows the labeling of ...
CyTOF Measurement of Immunocompetence Across Major Immune Cell Types [0.03%]
基于免疫细胞类型的免疫能力的 CyTOF 衡量方法
Priyanka B Subrahmanyam,Holden T Maecker
Priyanka B Subrahmanyam
The central role of the immune system is becoming appreciated in a wide variety of diseases. Cancer immunotherapy is one area that has yielded much recent success, although not all patients benefit equally. At the same time, recent studies ...
Vinko Tosevski,Egor Ulashchik,Andrea Trovato et al.
Vinko Tosevski et al.
Novel cell analyzers, including polychromatic flow cytometers and isotopical cytometry by time of flight (CyTOF) mass cytometers, enable simultaneous measurement of virtually bondless characteristics at the single-cell level. BrdU assays fo...
Measurement of Drug-Stabilized Topoisomerase II Cleavage Complexes by Flow Cytometry [0.03%]
流式细胞术测定药物稳定拓扑异构酶II切割复合物的方法
Marcelo de Campos Nebel,Micaela Palmitelli,Marcela González-Cid
Marcelo de Campos Nebel
The poisoning of Topoisomerase II (Top2) has been found to be useful as a therapeutic strategy for the treatment of several tumors. The mechanism of Top2 poisons involves a drug-mediated stabilization of a Top2-DNA complex, termed Top2 clea...
Detection and Quantification of Mitochondrial Fusion Using Imaging Flow Cytometry [0.03%]
利用影像流式细胞术检测和量化线粒体融合现象
Aldo Nascimento,Joanne Lannigan,David Kashatus
Aldo Nascimento
Mitochondria are dynamic organelles that perform several vital cellular functions. Requisite for these functions are mitochondrial fusion and fission. Despite the increasing importance of mitochondrial dynamics in a range of cellular proces...
Detection of Extracellular Vesicles Using Proximity Ligation Assay with Flow Cytometry Readout-ExoPLA [0.03%]
基于流式细胞术的邻近连接技术检测外泌体
Liza Löf,Linda Arngården,Tonge Ebai et al.
Liza Löf et al.
Extracellular vesicles (EVs) are continuously released by most cells, and they carry surface markers of their cells of origin. Found in all body fluids, EVs function as conveyers of cellular information, and evidence implicates them as mark...