Construction and screening of antigen targeted immune yeast surface display antibody libraries [0.03%]
靶向抗原免疫酵母展示抗体库的构建与筛选
Keith D Miller,Noah B Pefaur,Cheryl L Baird
Keith D Miller
These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain ant...
Methods and applications of laser-enabled analysis and processing (LEAP) [0.03%]
激光驱动分析和加工（LEAP）的方法及应用
Nan Lin,Jennifer R Cresswell,Genova A Richardson et al.
Nan Lin et al.
The LEAP (Laser-Enabled Analysis and Processing) platform combines in situ imaging with laser manipulation to efficiently identify, purify, and monitor expansion of high secreting clones. It also allows for rapid analysis of cell population...
Mara R Diaz,Sherry A Dunbar,James W Jacobson
Mara R Diaz
Diagnoses of opportunistic mycotic infections constitute an increasing clinical problem. Conventional diagnostic tests are time consuming and lack specificity and sensitivity for accurate and timely prognoses. This unit provides a comprehen...
Flow cytometric FRET analysis of ErbB receptor tyrosine kinase interaction [0.03%]
流式细胞仪上检测ErbB受体酪氨酸激酶相互作用的FRET分析法
Simone Diermeier-Daucher,Gero Brockhoff
Simone Diermeier-Daucher
The homologous and heterologous interaction of members of the epidermal growth factor (EGF)-related receptor tyrosine kinase (RTK) family (ErbB or HER family receptors) upon ligand binding is the initial key event in signal transduction by ...
Use of spectral fluorescence resonance energy transfer to detect nitric oxide-based signaling events in isolated perfused lung [0.03%]
应用光谱荧光共振能量转移检测孤立灌流肺中的基于一氧化氮的信号事件
Claudette M St Croix,Eileen M Bauer
Claudette M St Croix
Fluorescence resonance energy transfer (FRET) is a fluorescence microscopy technique suitable for live cells and capable of detecting changes in the conformational state of a single protein or the distance between two interacting proteins w...
From in vitro to in vivo: imaging from the single cell to the whole organism [0.03%]
从体外到体内：从单细胞到整体动物的成像技术
Jung Julie Kang,Ildiko Toma,Arnold Sipos et al.
Jung Julie Kang et al.
This unit addresses the applications of fluorescence microscopy and quantitative imaging to study multiple physiological variables of living tissue. Protocols are presented for fluorescence-based investigations ranging from in vitro cell an...
Paul S Maddox
Paul S Maddox
Imaging of subcellular events has been key to recent advances in the basic knowledge base as well as drug discovery for disease states such as cancer. In a snowball effect, advances in imaging have spurred new investigations, which have inc...
Helen M Cooper,Yvonne Paterson
Helen M Cooper
Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large q...
Helena Harlin,Thomas F Gajewski
Helena Harlin
Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphoryla...