Michael D Ward,Gregory Kaduchak
Michael D Ward
Acoustic cytometry uses radiation pressure forces instead of or in addition to hydrodynamic focusing to position cells or particles in a flowing stream for analysis. Commercial implementations to date combine both hydrodynamic and acoustic ...
A Practical Cryopreservation and Staining Protocol for Immunophenotyping in Population Studies [0.03%]
适用于人群免疫分型研究的样品冷冻保存和染色方案
Helene Barcelo,Jessica Faul,Eileen Crimmins et al.
Helene Barcelo et al.
Large population-based cohort studies, through their prospective collection of a broad range of health information, represent an invaluable resource for novel insights into the pathogenesis of human diseases. Collection and cryopreservation...
Michael Muntifering,Daniel Castranova,Gregory A Gibson et al.
Michael Muntifering et al.
Biologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation of tissues for optical microscopy often involves sectioning to a thickness of 50-100 µm, the practical limits of...
Resolution of Viable and Membrane-Compromised Free Bacteria in Aquatic Environments by Flow Cytometry [0.03%]
流式细胞术在水环境中分辨活的和膜受损的游离细菌中的应用
Gérald Grégori,Michel Denis,Sergio Sgorbati et al.
Gérald Grégori et al.
In aquatic environments, free heterotrophic bacteria play an extremely important role due to their high biomass, wide panel of metabolisms, and ubiquity, as well as the toxicity of certain species. This unit presents a nucleic-acid double-s...
Claire E J Cheetham
Claire E J Cheetham
Multiphoton intravital calcium imaging is a powerful technique that enables high-resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of...
Joseph R Daniele,Kartoosh Heydari,Andrew Dillin
Joseph R Daniele
Healthy, functional mitochondria are central to many cellular and physiological phenomena, including aging, metabolism, and stress resistance. A key feature of healthy mitochondria is a high membrane potential (Δψ) or charge differential ...
Peter O Bayguinov,Dennis M Oakley,Chien-Cheng Shih et al.
Peter O Bayguinov et al.
Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three-dimensional structural studies of biological cells and tissues...
James C S Wood
James C S Wood
A number of methods have been developed to compare single parameter histograms. Some perform a channel-by-channel analysis and others give a single statistic about how the histograms may or may not differ. If they do differ, then the signif...
Comparative Study
Current protocols in cytometry. 2018 Jan 18:83:10.20.1-10.20.20. DOI:10.1002/cpcy.33 2018
Generating Quantitative Cell Identity Labels with Marker Enrichment Modeling (MEM) [0.03%]
基于标记物丰富度建模的生成定量细胞身份标签的方法
Kirsten E Diggins,Jocelyn S Gandelman,Caroline E Roe et al.
Kirsten E Diggins et al.
Multiplexed single-cell experimental techniques like mass cytometry measure 40 or more features and enable deep characterization of well-known and novel cell populations. However, traditional data analysis techniques rely extensively on hum...
Howard M Shapiro,William G Telford
Howard M Shapiro
Lasers are the principal light sources for flow cytometers. Virtually all cytometers are equipped with at least one (and often many more) lasers. This unit covers the various types of lasers available and the qualities that make them suitab...