Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry [0.03%]
多参数流式细胞术监测多发性骨髓瘤可测量的残留病灶
Kah Teong Soh,Paul K Wallace
Kah Teong Soh
Recent interest in high sensitivity multiple myeloma (MM) measurable residual disease (MRD) testing is a direct consequence of the high-quality responses achieved using novel therapeutic agents and better treatment strategies. Traditional d...
Detection of Histone H2AX Phosphorylation on Ser-139 as an Indicator of DNA Damage [0.03%]
组蛋白H2AX第139位丝氨酸磷酸化作为DNA损伤标志的检测方法
Hong Zhao,Xuan Huang,H Dorota Halicka et al.
Hong Zhao et al.
This unit describes immunocytochemical detection of histone H2AX phosphorylated on Ser-139 (γH2AX) to reveal DNA damage, particularly when the damage involves the presence of DNA double-strand breaks (DSBs). These breaks often result from ...
Flow Cytometry Analysis of Free Intracellular NAD+ Using a Targeted Biosensor [0.03%]
利用靶向型生物传感器进行自由胞内NAD+的流式细胞术分析
Jared M Eller,Melissa L Stewart,Alexandria J Slepian et al.
Jared M Eller et al.
Flow cytometry approaches combined with a genetically encoded targeted fluorescent biosensor are used to determine the subcellular compartmental availability of the oxidized form of nicotinamide adenine dinucleotide (NAD+ ). The availabilit...
Martin Büscher
Martin Büscher
The term flow cytometry, used since the seventies, describes a technology employed mainly in biology and medicine to measure and classify suspended particles, e.g., cells or microspheres. Measurable cell parameters include: geometric proper...
Nithianandan Selliah,Steven Eck,Cherie Green et al.
Nithianandan Selliah et al.
Analytical method validation provides a means to ensure that data are credible and reproducible. This unit will provide a brief introduction to analytical method validation as applied to cellular analysis by flow cytometry. In addition, the...
Multiphoton Imaging of Collagen, Elastin, and Calcification in Intact Soft-Tissue Samples [0.03%]
完整软组织样品中的胶原蛋白、弹性蛋白和钙化多光子成像
Piyusha S Gade,Anne M Robertson,Chih-Yuan Chuang
Piyusha S Gade
Multiphoton-induced second-harmonic generation and two-photon excitation enable imaging of collagen and elastin fibers at micron-level resolution to depths of hundreds of microns, without the use of exogenous stains. These attributes can be...
Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry [0.03%]
多色流式细胞术分析造血干细胞和祖细胞的细胞周期
Amy Galvin,Meredith Weglarz,Kat Folz-Donahue et al.
Amy Galvin et al.
Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self-renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investig...
Flow Cytometric Monitoring for Residual Disease in B Lymphoblastic Leukemia Post T Cell Engaging Targeted Therapies [0.03%]
靶向治疗后B淋巴母细胞白血病残留病的流式检测监测
Sindhu Cherian,Maryalice Stetler-Stevenson
Sindhu Cherian
The use of targeted therapy is growing in the setting of hematopoietic neoplasms. Flow cytometry is a cornerstone of residual disease monitoring post therapy in this group of malignancies. Often, there is overlap between antigens targeted b...
Deriving Extracellular Vesicle Size From Scatter Intensities Measured by Flow Cytometry [0.03%]
从流式细胞仪测量的散射强度中推导出胞外囊泡的大小
Leonie de Rond,Frank A W Coumans,Rienk Nieuwland et al.
Leonie de Rond et al.
Flow cytometry is commonly used to investigate the potential for extracellular vesicles (EVs) to be biomarkers of disease. A typical flow cytometer detects fluorescence and scatter intensities of single EVs in arbitrary units. These arbitra...
J Paul Robinson
J Paul Robinson
Although in recent years flow cytometry has become commonplace in hematology and immunology laboratories, application of the technology to microbiology remains largely unrealized. This overview presents the historical background, discusses ...