Training Novices in Generation and Analysis of High-Dimensional Human Cell Phospho-Flow Cytometry Data [0.03%]
蛋白质磷酸化流式细胞术数据的生成与分析入门培训
Caroline E Roe,Madeline J Hayes,Sierra M Barone et al.
Caroline E Roe et al.
This article presents a single experiment designed to introduce a trainee to multiple advanced bench and analysis techniques, including high-dimensional cytometry, profiling cell signaling networks, functional assays with primary human tiss...
Panel Design and Optimization for High-Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry [0.03%]
光谱流式细胞仪高维免疫表型检测的 PANEL 设计与优化
Laura Ferrer-Font,Christophe Pellefigues,Johannes U Mayer et al.
Laura Ferrer-Font et al.
Technological advances in fluorescence flow cytometry and an ever-expanding understanding of the complexity of the immune system have led to the development of large (20+ parameters) flow cytometry panels. However, as panel complexity and s...
Single-Cell Analysis of Cytokine mRNA and Protein Expression by Flow Cytometry [0.03%]
流式细胞仪检测细胞因子mRNA和蛋白表达的单细胞分析方法
Rubina Pal,Jayne Schaubhut,Darcey Clark et al.
Rubina Pal et al.
Understanding how immune cells respond to external stimuli such as pathogens or drugs is a key component of biomedical research. Critical to the immune response are the expression of cell-surface receptors and the secretion of cytokines, wh...
Michael A Model
Michael A Model
Cell volume is an important parameter in studying cell adaptation to anisosmotic stress, activation of monovalent ion channels, and cell death. This article describes a method for measurement of the volumes of adherent cells using a standar...
Enumeration of Fetal Red Blood Cells, Hemoglobin-Specific RBC Cells, and F Reticulocytes in Human Blood [0.03%]
人血液中胎儿红细胞、血红蛋白特异性RBC细胞和F网织红细胞的分类计数
Bruce H Davis
Bruce H Davis
Recent advances in analytical cytometry have improved diagnostic tools for the study of erythropoiesis in anemic patients and resolution of differential diagnosis in diseases of the erythron. This article presents three applications of red ...
Amicia D Elliott
Amicia D Elliott
In light microscopy, illuminating light is passed through the sample as uniformly as possible over the field of view. For thicker samples, where the objective lens does not have sufficient depth of focus, light from sample planes above and ...
Immunophenotypic Detection of Measurable Residual (Stem Cell) Disease Using LAIP Approach in Acute Myeloid Leukemia [0.03%]
急性髓系白血病采用LAIP方法检测免疫表型残留(CD34+)病态干细胞的研究
Wendelien Zeijlemaker,Angele Kelder,Jacqueline Cloos et al.
Wendelien Zeijlemaker et al.
Half of the patients with acute myeloid leukemia (AML), who achieve complete remission after chemotherapy treatment, will ultimately experience a relapse. Measurable residual disease (MRD) is an important post-treatment risk factor in AML, ...
Assessment of DNA Susceptibility to Denaturation as a Marker of Chromatin Structure [0.03%]
DNA变性敏感性的评估作为一种染色质结构标志的评价
Zbigniew Darzynkiewicz,Dorota H Halicka,Hong Zhao et al.
Zbigniew Darzynkiewicz et al.
The susceptibility of DNA in situ to denaturation is modulated by its interactions with histone and nonhistone proteins, as well as with other chromatin components related to the maintenance of the 3D nuclear structure. Measurement of DNA p...
Simultaneous Polychromatic Immunofluorescent Staining of Tissue Sections and Consecutive Imaging of up to Seven Parameters by Standard Confocal Microscopy [0.03%]
组织切片的多色免疫荧光同时染色及激光扫描共聚焦显微镜连续成像多达七组参数的方法
Alfonso J Schmidt,Johannes U Mayer,Paul K Wallace et al.
Alfonso J Schmidt et al.
Confocal microscopy has been an important imaging tool for life scientists for over 20 years. Early techniques focused on indirect staining processes that involved staining with an unconjugated primary antibody, followed by incubation with ...
Aleksandra Klimas,Brendan Gallagher,Yongxin Zhao
Aleksandra Klimas
Optical imaging techniques are often used in neuroscience to understand brain function and discern disease pathogenesis. However, the optical diffraction limit precludes conventional optical imaging approaches from resolving nanoscopic stru...