Jessica P Houston,Mark A Naivar,Patrick Jenkins et al.
Jessica P Houston et al.
In flow cytometry, the fluorescence decay time of an excitable species has been largely underutilized and is not likely found as a standard parameter on any imaging cytometer, sorting, or analyzing system. Most cytometers lack fluorescence ...
Hannah Chen,Stephen H Thorne
Hannah Chen
Traditional approaches for translating observations of molecular events into the context of a living organism have suffered from the requirements for either sacrificing animals at multiple time points prior to labor-intensive analyses of mu...
Confocal microscopy for high-resolution and high-content analysis of the cell cycle [0.03%]
用于细胞周期高分辨率和高内涵分析的共聚焦显微镜技术
Laura Furia,Piergiuseppe Pelicci,Mario Faretta
Laura Furia
Optical fluorescence microscopy offers a wide range of technological solutions to address many questions in biomedical research. Spatial resolution has been greatly improved by the use of confocal microscopes, providing a 3-D analysis of th...
Laura Furia,Piergiuseppe Pelicci,Mario Faretta
Laura Furia
One of the major limitations of flow cytometry (FCM) is the absence of an intracellular view. Automated microscopy and image analysis, together with technological developments, led to new approaches in cytometry that bypass the above limita...
FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells [0.03%]
激光扫描细胞计数仪在附着细胞的大规模群体中的FRET成像
Quang-Minh Doan-Xuan,Nikoletta Szalóki,Katalin Tóth et al.
Quang-Minh Doan-Xuan et al.
The application of FRET (fluorescence resonance energy transfer) sensors for monitoring protein-protein interactions under vital conditions is attracting increasing attention in molecular and cell biology. Laser-scanning cytometry (LSC), a ...
Randall T Schirra Jr,Peijun Zhang
Randall T Schirra Jr
Correlative fluorescence and electron microscopy (CFEM) is a multimodal technique that combines dynamic and localization information from fluorescence methods with ultrastructural data from electron microscopy, to give new information about...
Attenuation of replication stress-induced premature cellular senescence to assess anti-aging modalities [0.03%]
通过减轻复制胁迫诱导的细胞衰老来评估抗衰老方法的作用机制
Hong Zhao,Zbigniew Darzynkiewicz
Hong Zhao
Described is an in vitro model of premature senescence in pulmonary adenocarcinoma A549 cells induced by persistent DNA replication stress in response to treatment with the DNA damaging drug mitoxantrone (Mxt). The degree of cellular senesc...
Application of flow-FISH for dynamic measurement of telomere length in cell division [0.03%]
流式荧光原位杂交技术(flow-FISH)在细胞分裂过程中测量端粒长度的应用研究
Vyacheslav I Borisov,Olga Y Korolkova,Vladimir S Kozhevnikov
Vyacheslav I Borisov
This method makes it possible to measure the fluorescence of a DNA probe in cells with known division number and targeted surface antigen. In fact, this method is a combination or consistent application of three other methods: cell tracking...
Application of the PrimRglo assay chemistry to multiplexed bead assays [0.03%]
PrimRglo化学在多重珠式测定中的应用
Liang Fang,Anna Weis,Lawrence Kurniawan Wong et al.
Liang Fang et al.
In this unit, we describe a multiplex microsphere quantitative PCR. The system is based on the use of two additional oligonucleotides within a single tube PCR reaction. The first oligonucleotide is modified with a single base pair mismatch ...
The application of KillerRed for acute protein inactivation in living cells [0.03%]
KillerRed在活细胞中用于急性蛋白质灭活的应用研究
Timothy S Jarvela,Adam D Linstedt
Timothy S Jarvela
Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivati...