Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS [0.03%]
利用nanoFACS检测并分选细胞外囊泡和病毒
Aizea Morales-Kastresana,Joshua A Welsh,Jennifer C Jones
Aizea Morales-Kastresana
Extracellular vesicles (EVs) are sub-micron-sized membranous spheres secreted by cells. EVs play a functional role as intercellular communicators and are associated with a number of diseases. Research into EVs is an area of growing interest...
Tomasz Brzoska,Tomasz W Kaminski,Margaret F Bennewitz et al.
Tomasz Brzoska et al.
Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion brought about by ventilation and cardiac contractions. A pr...
Small Particle Fluorescence and Light Scatter Calibration Using FCMPASS Software [0.03%]
使用FCMPASS软件进行小颗粒荧光和散射光校准
Joshua A Welsh,Jennifer C Jones
Joshua A Welsh
Use of flow cytometry to analyze small particles has been implemented for several decades. More recently, small particle analysis has become increasingly utilized owing to the increased sensitivity of conventional and commercially available...
Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue [0.03%]
优化的随机光学重建显微镜用于病理组织染色质结构成像
Jianquan Xu,Hongqiang Ma,Yang Liu
Jianquan Xu
Direct visualization of higher-order chromatin structure at the molecular scale is of great importance for understanding the impact of chromatin organization on gene expression in many biological processes. Understanding the changes in chro...
Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood [0.03%]
全血中性粒细胞碱性磷酸酶流式细胞术定量检测
Jorge Bardina,Laura G Rico,Michael D Ward et al.
Jorge Bardina et al.
Translational research has improved the diagnosis and follow-up of hematological diseases and malignancies. However, some classical diagnostics used for research and clinical practice that have remain practically unchanged for decades may b...
Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens [0.03%]
冠状病毒病临床标本的采集、处理及灭活指南
William Bain,Janet S Lee,Alan M Watson et al.
William Bain et al.
SARS-CoV-2 is a novel coronavirus that causes the acute respiratory disease-Coronavirus disease 2019 (COVID-19)-which has led to a global health crisis. Currently, no prophylactics or therapies exist to control virus spread or mitigate the ...
Amy Fox,Taru S Dutt,Burton Karger et al.
Amy Fox et al.
Flow cytometry allows the visualization of physical, functional, and/or biological properties of cells including antigens, cytokines, size, and complexity. With increasingly large flow cytometry panels able to analyze up to 50 parameters, t...
Automated Motion Tracking and Data Extraction for Red Blood Cell Biomechanics [0.03%]
红细胞 biomechanics的自动运动追踪和数据提取技术
Arun Kumar,Brendan R Schmidt,Zyrina Alura C Sanchez et al.
Arun Kumar et al.
Red blood cell biomechanics can provide us with a deeper understanding of macroscopic physiology and have the potential of being used for diagnostic purposes. In diseases like sickle cell anemia and malaria, reduced red blood cell deformabi...
Acute Myeloid Leukemia Minimal Residual Disease Detection: The Difference from Normal Approach [0.03%]
急性髓系白血病微小残留病检测：与常规方法的不同之处
Brent L Wood
Brent L Wood
The identification of residual leukemia following therapy, termed minimal or measurable residual disease (MRD), has emerged as one of the most important prognostic factors for patients with acute leukemia, including acute myeloid leukemia (...
Hazel Davey,Stéphane Guyot
Hazel Davey
For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold standard) usage equates viability and culturability (i.e., the ability to multiply) b...