Synthesis of 4'-Selenoribonucleosides, the Building Blocks of 4'-SelenoRNA, Using a Hypervalent Iodine [0.03%]
使用高价碘合成4'-硒核糖核苷，作为构建4'-硒RNA的构件
Noriko Saito-Tarashima,Masashi Ota,Noriaki Minakawa
Noriko Saito-Tarashima
Herein is described a detailed protocol for the synthesis of 4'-selenoribonucleoside derivatives that involves the use of a hypervalent iodine species. These derivatives are versatile units for the preparation of 4'-selenoRNA. Large-scale s...
Quantitative Analysis of Nucleic Acid Stability with Ligands Under High Pressure to Design Novel Drugs Targeting G-Quadruplexes [0.03%]
高压下核酸配体稳定性的定量分析以设计针对G-四链体的新药
Shuntaro Takahashi,Naoki Sugimoto
Shuntaro Takahashi
Nucleic acids (DNA and RNA) can form various non-canonical structures. Because some serious diseases are caused by the conformational change of G-quadruplex DNA structures, the development of ligands that bind and stabilize G-quadruplex DNA...
Nucleoside-O-Methyl-(H)-Phosphinates: Novel Monomers for the Synthesis of Methylphosphonate Oligonucleotides Using H-Phosphonate Chemistry [0.03%]
核苷-O-甲基-(氢)磷酸酯：用于合成甲基膦酸寡核苷酸的新型单体（使用氢膦化学）
Ondřej Kostov,Ondřej Páv,Ivan Rosenberg
Ondřej Kostov
This unit comprises the straightforward synthesis of protected 2'-deoxyribonucleoside-O-methyl-(H)-phosphinates in both 3'- and 5'-series. These compounds represent a new class of monomers compatible with the solid-phase synthesis of oligon...
Sarah A Woodson
Sarah A Woodson
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are ...
Solid-Phase Synthesis of RNA Analogs Containing Phosphorodithioate Linkages [0.03%]
含磷二硫化链的RNA类似物固相合成
Xianbin Yang
Xianbin Yang
The oligoribonucleotide phosphorodithioate (PS2-RNA) modification uses two sulfur atoms to replace two non-bridging oxygen atoms at an internucleotide phosphorodiester backbone linkage. Like a natural phosphodiester RNA backbone linkage, a ...
Vibration Ball Milling for the Synthesis of 5'-Thioadenosine 5'-Pyrophosphate (P'→5') Adenosine (dASppA) [0.03%]
振动球磨法合成5'-硫代腺苷-5'-焦磷酸(dASppA)
Olga Eguaogie,Joseph S Vyle
Olga Eguaogie
Using vibration ball milling, 5'-chloro-5'-deoxyadenosine (CldA) reacts cleanly with 4-methoxybenzyl mercaptan (MobSH), under basic conditions, to the corresponding thioether (MobSdA), which is isolated following precipitation and triturati...
Synthesis of Oligodeoxynucleotides Containing a C8-2'-Deoxyguanosine Adduct Formed by the Carcinogen 3-Nitrobenzanthrone [0.03%]
含有致癌剂3-硝基苯并蒽酮形成的C8-2'-脱氧鸟苷加合物的寡去氧核糖核酸的合成
Arindom Chatterjee,Chanchal K Malik,Ashis K Basu
Arindom Chatterjee
This unit describes the detailed procedure in five parts for the synthesis of the C8-2'-deoxyguanosine-3-aminobenzanthrone adduct located in a desired site in an oligonucleotide. The synthesis of the protected 2'-deoxyguanosine, O6 -benzyl-...
Geeta Meher,Nabin K Meher,Radhakrishnan P Iyer
Geeta Meher
Oligonucleotides carrying a variety of chemical modifications including conjugates are finding increasing applications in therapeutics, diagnostics, functional genomics, proteomics, and as research tools in chemical and molecular biology. T...
Carbocyclic C-C Bond Formation: Intramolecular Radical Ring Closure to Yield Diastereomerically Pure (7'S-Me- or 7'R-Me-) Carba-LNA Nucleotide Analogs [0.03%]
碳碳键形成：生成对映纯（7′S-甲基或7′R-甲基）卡巴-LNA核苷酸类似物的分子内自由基环化反应
Oleksandr Plashkevych,Ram Shankar Upadhayaya,Jyoti Chattopadhyaya
Oleksandr Plashkevych
In light of the impressive gene-silencing properties of carba-LNA modified oligo DNA and RNA, both in antisense RNA and siRNA approaches, which have been confirmed as proof-of-concept for biochemical applications in post-transcriptional gen...
Engineered Polymerases with Altered Substrate Specificity: Expression and Purification [0.03%]
底物特异性改变的工程化聚合酶：表达和纯化
Ali Nikoomanzar,Matthew R Dunn,John C Chaput
Ali Nikoomanzar
Polymerase engineering is making it possible to synthesize xeno-nucleic acid polymers (XNAs) with diverse backbone structures and chemical functionality. The ability to copy genetic information back and forth between DNA and XNA has led to ...