Line Dahl Poulsen,Jeppe Vinther
Line Dahl Poulsen
RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part o...
Recording and Analyzing Nucleic Acid Distance Distributions with X-Ray Scattering Interferometry (XSI) [0.03%]
利用X射线衍射干涉仪(XSI)记录和分析核酸距离分布
Thomas Zettl,Rhiju Das,Pehr A B Harbury et al.
Thomas Zettl et al.
Most structural techniques provide averaged information or information about a single predominant conformational state. However, biological macromolecules typically function through series of conformations. Therefore, a complete understandi...
Yumeng Hao,Jen Bohon,Ryan Hulscher et al.
Yumeng Hao et al.
RNA footprinting by hydroxyl radical cleavage provides 'snapshots' of RNA tertiary structure or protein interactions that bury the RNA backbone. Generation of hydroxyl radicals with a high-flux synchrotron X-ray beam provides analysis on a ...
Synthesis of Chimeric Oligonucleotides Having Modified Internucleotide Linkages via an Automated H-Phosphonate/Phosphoramidite Approach [0.03%]
通过自动化H-磷腈/磷酸酯方法合成含有修饰的核苷酸间连接体的嵌合型寡核苷酸
Danielle Vlaho,Masad J Damha
Danielle Vlaho
This article describes an automated solid-phase approach for the synthesis of chimeric oligonucleotides containing phosphoramidate-modified internucleotide linkages. An optimized H-phosphonate synthetic cycle is combined with the commonly u...
Synthesis of β-Nicotinamide Riboside Using an Efficient Two-Step Methodology [0.03%]
一种高效的两步法合成β-烟酰胺单核苷酸的方法学研究
Ning Zhang,Anthony A Sauve
Ning Zhang
A two-step chemical method for the synthesis of β-nicotinamide riboside (NR) is described. NR has achieved wide use as an NAD+ precursor (vitamin B3) and can significantly increase central metabolite NAD+ concentrations in mammalian cells....
Synthesis of Bipartite Tetracysteine PNA Probes for DNA In Situ Fluorescent Labeling [0.03%]
用于DNA原位荧光标记的双元四半胱氨酸PNA探针的合成
Ge-Min Fang,Oliver Seitz
Ge-Min Fang
"Label-free" fluorescent probes that avoid additional steps or building blocks for conjugation of fluorescent dyes with oligonucleotides can significantly reduce the time and cost of parallel bioanalysis of a large number of nucleic acid sa...
Practical Synthesis of 4'-Thioribonucleosides from L-Arabinose via Novel Reductive Ring-Contraction Reaction and Pummerer-Type Thioglycosylation [0.03%]
由L-阿拉伯糖合成4'-硫核苷的新型还原缩环和Pummerer型硫苷化反应方法
Hideaki Wakamatsu,Kozue Nitta,Nozomi Shoji et al.
Hideaki Wakamatsu et al.
The detailed practical synthesis of 4'-thionucleosides starting from L-arabinose is described here. 1,4-Anhydro-2,3-O-isopropylidene-4-thioribitol, which is the key intermediate for the synthesis of 4'-thionucleosides, is obtained from L-ar...
3'-O-Caged 2'-Deoxynucleoside Triphosphates for Light-Mediated, Enzyme-Catalyzed, Template-Independent DNA Synthesis [0.03%]
用于光介导、酶催化和模版无关的DNA合成的3'-O-Skin 2'-去氧核糖核酸三磷酸
Anu Stella Mathews,Haikang Yang,Carlo Montemagno
Anu Stella Mathews
Synthesis, purification, and characterization of 3'-O-caged 2'-deoxyribonucleoside triphosphates (dNTPs), namely 3'-O-(2-nitrobenzyl)-2'-deoxy ribonucleoside triphosphates (NB-dNTPs) and 3'-O-(4,5-dimethoxy-2-nitrobenzyl)-2'-deoxy ribonucle...
Palladium-Catalyzed Synthesis of (E)-5-(3-Aminoallyl)-Uridine-5'-O-Triphosphates [0.03%]
钯催化合成(E)-5-(3-氨基亚烯丙基)尿苷-5'-三磷酸盐
Muthian Shanmugasundaram,Annamalai Senthilvelan,Anilkumar R Kore
Muthian Shanmugasundaram
This unit describes a simple, reliable, and efficient chemical method for the synthesis of 5-(3-aminoallyl)-2'-deoxyuridine-5'-O-triphosphate (AA-dUTP) and 5-(3-aminoallyl)-uridine-5'-O-triphosphate (AA-UTP), starting from the corresponding...
Yusuke Maeda,Nazuki Niwa,Yoshihito Ueno
Yusuke Maeda
Benzene-glycol nucleic acid-DNA chimeras form thermally and thermodynamically stable duplexes with complementary RNAs, and have base-discriminating abilities. This unit describes the synthesis of four nucleoside analogs, an adenine, cytosin...