An Improved Strategy for the Chemical Synthesis of 3',5'-Cyclic Diguanylic Acid [0.03%]
改进的化学合成环二鸟苷酸策略
Andrzej Grajkowski,Mayumi Takahashi,Tomasz Kaczyński et al.
Andrzej Grajkowski et al.
The physiological functions of c-di-GMP and its involvement in many key processes led to its recognition as a major and ubiquitous bacterial second messenger. Aside from being a bacterial signaling molecule, c-di-GMP is also an immunostimul...
Xiao-Nan Zhang,Zhefu Dai,Qinqin Cheng et al.
Xiao-Nan Zhang et al.
This chemoenzymatic procedure describes a strategy for the preparation of 4'-thioribose nicotinamide adenine dinucleotide (S-NAD+ ), including chemical synthesis of nicotinamide 4'-riboside (S-NR), recombinant expression and purification of...
Liquid-Phase Oligonucleotide Synthesis: Past, Present, and Future Predictions [0.03%]
寡核苷酸的液相合成：过去、现在和未来预测
Alejandro Gimenez Molina,Yogesh S Sanghvi
Alejandro Gimenez Molina
Therapeutic oligonucleotides have emerged as a powerful paradigm with the ability to treat a wide range of the human diseases. As a result, we have witnessed more than one hundred oligonucleotides currently in active clinical trials and eig...
Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use [0.03%]
并行钳和多嘌呤发夹（PPRH）用于基因沉默及三链亲和捕获：设计、合成及应用
Anna Aviñó,Ramon Eritja,Carlos J Ciudad et al.
Anna Aviñó et al.
Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols...
Synthesis of 2'-deoxy-4-aminopyridinylpseudocytidine Derivatives for Incorporation Into Triplex Forming Oligonucleotides [0.03%]
三链形成寡核苷酸中掺入2'-脱氧-4-氨基吡啶基伪胞苷类似物的设计、合成与应用研究
Yosuke Taniguchi,Lei Wang,Hidenori Okamura et al.
Yosuke Taniguchi et al.
This unit describes the detailed synthetic protocol for the preparation of the phosphoramidite units of the 2'-deoxy-4-aminopyridinylpseudocytidine derivatives. These C-nucleoside derivatives are useful units for the incorporation into trip...
An Efficient Synthesis of 5-Aminopropargyl-Pyrimidine-5'-O-Triphosphates Through Palladium-Catalyzed Sonogashira Coupling [0.03%]
钯催化偶联高效构建5-炔丙氨基核苷酸内标物的方法学研究
Muthian Shanmugasundaram,Annamalai Senthilvelan,Anilkumar R Kore
Muthian Shanmugasundaram
The utilization of 5-aminopropargyl nucleotide serves as a versatile molecular biology tool for the introduction of functional groups into a nucleic acid target of interest by using in-vitro enzymatic incorporation method. This article desc...
Synthesis of RNA Crosslinking Oligonucleotides Modified with 2-Amino-7-Deaza-7-Propynyl-6-Vinylpurine [0.03%]
含2-氨基-7-脱氧-7-丙炔基-6-乙烯基嘌呤修饰的RNA交联寡核苷酸的合成
Ken Yamada,Yusuke Abe,Fumi Nagatsugi
Ken Yamada
This article describes procedures to synthesize 2'-OMe-RNA modified with cross-linkable 2-amino-7-deaza-7-propynyl-6-vinylpurine (ADpVP) and preparation of the RNA-crosslinking experiment in vitro. All synthesis steps yield the desired comp...
Affinity Isolation of Defined Genomic Fragments Cleaved by Nuclease S1-based Artificial Restriction DNA Cutter [0.03%]
基于核酸酶S1的体内定点人工限制性内切酶及大片段基因组DNA连续自动降解体系的研究
Arivazhagan Rajendran,Narumi Shigi,Jun Sumaoka et al.
Arivazhagan Rajendran et al.
The human genome is highly susceptible to various modifications, lesions, and damage. To analyze lesions and proteins bound to a defined region of the human genome, the genome should be fragmented at desired sites and the region of interest...
Synthesis of 2-Amino-4-Fluoropyridine-C-Nucleoside Phosphoramidite for Incorporation into Oligonucleotides [0.03%]
用于寡核苷酸中包含的2-氨基-4-氟吡啶-c-核糖核酸基磷酰胺
Kousuke Sato,Akira Matsuda
Kousuke Sato
Straightforward and efficient methods for the synthesis of 2-amino-4-fluoropyridine-C-nucleoside (dF P) and the solid-phase synthesis of oligodeoxynucleotides containing dF P using a phosphoramidite are described. The synthesis of dF P is a...
6-Phenylpyrrolocytidine: An Intrinsically Fluorescent, Environmentally Responsive Nucleoside Analogue [0.03%]
6-苯基吡咯核苷类似物：固有荧光及环境响应的核苷类似物
Sung Ju Cho,Arash Ghorbani-Choghamarani,Yoshio Saito et al.
Sung Ju Cho et al.
The detailed synthetic protocols for the preparation of phosphoramidite reagents compatible with standard, automated oligonucleotide synthesis for the 2'-deoxy- and ribo-6-phenylpyrrolocyitidine are reported. Each protocol starts with the p...