J R Thayer,Srinivasa Rao,Nitin Puri
J R Thayer
Formation of aberrant 2'-5' linkages can unintentionally occur in chemical synthesis of RNA. These linkages may arise by phosphoryl migration during deprotection, release, or subsequent steps during manufacture of therapeutic RNA. Their pre...
Synthesis of a 4-selenothymidine phosphoramidite and incorporation into oligonucleotides [0.03%]
4-硒胸苷磷酰胺苷的合成及其引入到寡核苷酸中
Jia Sheng,Zhen Huang
Jia Sheng
The detailed synthetic protocol for a 4-selenothymidine phosphoramidite and its use to prepare modified oligonucleotides is described here. The Se-phosphoramidite synthesis was achieved by developing a useful protection and deprotection sys...