Solid-Phase Synthesis of DNA and RNA 5'-O-Triphosphates Using cycloSal Chemistry [0.03%]
固相循环萨尔化学合成DNA和RNA 5'-O-三磷酸
Ivo Sarac,Chris Meier
Ivo Sarac
A chemical method for the synthesis of short strands of DNA and RNA 5'-O-triphosphates is described that makes use of the conventional coupling and oxidizing reagents that are readily available on standard DNA/RNA synthesizers. After automa...
Hans A V Kistemaker,Nico J Meeuwenoord,Herman S Overkleeft et al.
Hans A V Kistemaker et al.
Solid-phase methodology for synthesis of adenosine diphosphate ribose oligomers (ADPr-oligomers) of defined length is described using an advanced 2-ribosyl adenosine phosphoramidite building block that is prepared in solution via an expedit...
Highly Regioselective Methylation of Guanosine Nucleotides: An Efficient Synthesis of 7-Methylguanosine Nucleotides [0.03%]
高区域选择性鸟苷核苷酸N7甲基化：7-甲基鸟苷高效合成的新方法
Annamalai Senthilvelan,Muthian Shanmugasundaram,Anilkumar R Kore
Annamalai Senthilvelan
This article describes a simple, reliable, efficient, and general method for the synthesis of 7-methylguanosine nucleotides such as 7-methylguanosine 5'-O-monophosphate (m7 GMP), 7-methylguanosine 5'-O-diphosphate (m7 GDP), 7-methyl-2'-deox...
Synthesis of N2 -Aryl-2'-Deoxyguanosine Modified Phosphoramidites and Oligonucleotides [0.03%]
N2-aryl-2'-脱氧鸟苷修饰的磷酰胺和寡核苷酸的合成
Pratibha P Ghodke,P I Pradeepkumar
Pratibha P Ghodke
The N2 -position of 2'-deoxyguanosine (N2 -position in dG) is well known for forming carcinogenic minor groove DNA adducts, which originate from environmental pollutants, chemicals, and tobacco smoke. The N2 -dG DNA adducts have strong impl...
Full Pre-Steady-State Kinetic Analysis of Single Nucleotide Incorporation by DNA Polymerases [0.03%]
单核苷酸聚合酶掺入的全预平衡态动力学分析
Marleen Renders,Jean-Marie Frère,Dominique Toye et al.
Marleen Renders et al.
By measuring a DNA polymerase incorporation reaction on a very short time scale (5 ms to 10 s) and with a high enzyme concentration, the isolated event of a single nucleotide incorporation can be analyzed. Practically, this is done using a ...
Synthesis of Monovalent N-Acetylgalactosamine Phosphoramidite for Liver-Targeting Oligonucleotides [0.03%]
用于肝脏靶向寡核苷酸的单价N-乙酰半乳糖胺磷酰胺苷的合成
Tsuyoshi Yamamoto,Chisato Terada,Koki Kashiwada et al.
Tsuyoshi Yamamoto et al.
Ligand-targeted drug delivery (LTDD) has emerged as an attractive option in the field of oligonucleotide drugs following the great success of N-acetylgalactosamine (GalNAc)-conjugated siRNA and antisense oligonucleotides. GalNAc is a well-k...
Construction of Pyrimidine Bases Bearing Carboxylic Acid Equivalents at the C5 Position by Postsynthetic Modification of Oligonucleotides [0.03%]
通过寡核苷酸的后合成修饰在C5位置构建含嘧啶碱基的羧酸衍生物
Yuta Ito,Kazuki Yamamoto,Yoshiyuki Hari
Yuta Ito
This unit describes postsynthetic modification of oligonucleotides (ONs) containing 2'-deoxy-5-trifluoromethyluridine and 2'-deoxy-5-trifluoromethylcytidine. In ONs, the trifluoromethyl group at the C5 position of pyrimidine bases is conver...
Synthesis of Poly(ADP-ribose) Monomer Containing 2'-O-α-D-Ribofuranosyl Adenosine [0.03%]
含有2'-O-α-D-核糖呋喃糖基腺苷的多聚（ADP-核糖）单体的合成
Sergey N Mikhailov,Mikhail S Drenichev,Vladimir E Oslovsky et al.
Sergey N Mikhailov et al.
In this article, the earlier reported procedure for the synthesis of 2'-O-β-D-ribofuranosyl nucleosides was extended to the synthesis of 2'-O-α-D-ribofuranosyl adenosine, a monomeric unit of poly(ADP-ribose). It consists in condensation o...
Synthesis of 5-Alkynyl Substituted 2'-Arabinosyl 2'-Halogenated Uridine Nucleosides [0.03%]
5-炔基取代的2'-卤代脱氧阿拉伯糖核苷的合成
David H Hilko,Laurent F Bornaghi,Sally-Ann Poulsen
David H Hilko
This unit describes the detailed preparation of 5-alkynyl-2'-halogenated arabinosyl uridine nucleosides (2'-halo-ara-EdU) from uridine. These compounds were synthesized as prospective chemical probes for the detection of DNA synthesis in pr...
Immobilization of DNA on Biosensing Devices with Nitrogen Mustard-Modified Linkers [0.03%]
氮芥改性的接头共价固定DNA用于生物传感装置
Takaaki Kurinomaru,Naoshi Kojima,Ryoji Kurita
Takaaki Kurinomaru
Immobilization of DNA is an important step in relation to DNA-based biosensors and bioassays with multiple applications. This unit describes synthesis and applications of novel bifunctional linker molecules containing nitrogen mustard and o...