Kacper Skakuj,Katherine E Bujold,Chad A Mirkin
Kacper Skakuj
This protocol describes a method based on iodine and a base as mild coupling reagents to synthetize deoxyribonucleic guanidines (DNGs)-oligodeoxynucleotide analogues with a guanidine backbone. DNGs display unique properties, such as high ce...
Pegylation of RNA Spiegelmers by a Novel Widely Applicable Two-Step Process for the Conjugation of Carboxylic Acids to Amino-Modified Oligonucleotides [0.03%]
一种新的适用于多种RNA Spiegelmers的PEG化方法：两步法连接聚乙二醇和氨基修饰寡核苷酸
Lucas Bethge,Stefan Vonhoff
Lucas Bethge
The reaction between N-hydroxy succinimide (NHS) ester-activated carboxylic acids and amino-modified nucleic acids is commonly used for the post-synthetic modification of oligonucleotides. Here, we report a two-step variation of the method ...
Alexander Ripp,Jyoti Singh,Henning J Jessen
Alexander Ripp
Nucleoside triphosphates (NTPs) are essential biomolecules involved in almost all biological processes, and their study is therefore critical to understanding cellular biology. Here, we describe a chemical synthesis suitable for obtaining b...
Synthesis of Azobenzene Derivative Phosphoramidites for Incorporation into Oligonucleotides [0.03%]
偶氮苯衍生物磷酰胺单体的合成及其嵌入到寡核苷酸中
Matthew L Hammill,Jean-Paul Desaulniers
Matthew L Hammill
This article contains the detailed synthesis and characterization protocols of azobenzene containing siRNAs, which have photoswitchable properties effectively controlled with light. First, the azobenzene scaffolds are synthesized via reduct...
Synthesis of DNA and RNA Oligonucleotides Containing a Dual-Purpose Selenium-Modified Fluorescent Nucleoside Probe [0.03%]
含硒修饰的双功能荧光核苷探针的DNA和RNA寡聚核苷酸合成
Ashok Nuthanakanti,Seergazhi G Srivatsan
Ashok Nuthanakanti
Development of efficient tools that would enable direct correlation of nucleic acid structure and recognition in solution and in solid state at atomic resolution is highly desired. In this context, we recently developed dual-purpose nucleos...
Synthesis of 9-(6-Deoxy-α-L-Talofuranosyl)-6-Methylpurine and 9-(6-Deoxy-β-D-Allofuranosyl)-6-Methylpurine Nucleosides [0.03%]
9-(6-脱氧-α-L-塔洛呋喃糖基)-6-甲基嘌呤和9-(6-脱氧-β-D-阿洛呋喃糖基)-6-甲基嘌呤核苷的合成
Abdalla E A Hassan,Reham A I Abou-Elkhair,Hend M Maaroof et al.
Abdalla E A Hassan et al.
6-Methylpurine (MeP) is a cytotoxic adenine analog that does not exhibit selectivity when administered systemically and could be very useful in a gene therapy approach to cancer treatment involving Escherichia coli purine nucleoside phospho...
Synthesis of 2'-Deoxyuridine Modified with a 3,5-Difluoro-4-Methoxybenzylidene Imidazolinone Derivative for Incorporation into Oligonucleotide Probes for Detection of HER2 Breast Cancer Marker [0.03%]
合成带有3,5-二氟-4-甲氧基苯并亚胺基异唑啉酮衍生物的2'-脱氧尿苷用于掺入抗HER2乳腺癌标记的寡核苷酸探针中
Abed Saady,Noam Y Steinman,Melissa Wojtyniak et al.
Abed Saady et al.
Nucleoside intercalator conjugates (NICs) describe an innovative methodology developed in our research group for preparation of fluorescence turn-on DNA hybridization probes targeting specific mRNA sequences (e.g., breast cancer markers). I...
Synthesis of 2'-Fluorinated Northern Methanocarbacyclic (2'-F-NMC) Nucleosides and Their Incorporation Into Oligonucleotides [0.03%]
2'-氟化北方甲基卡宾环核糖（2'-F-NMC）核苷的合成及其在寡核苷酸中的引入
Masaaki Akabane-Nakata,Pawan Kumar,Namrata D Erande et al.
Masaaki Akabane-Nakata et al.
This article describes chemical synthesis of 2'-fluorinated Northern methanocarbacyclic (2'-F-NMC) nucleosides and phosphoramidites, based on a bicyclo[3.1.0]hexane scaffold bearing all four natural nucleobases (U, C, A, and G), and their i...
Synthesis of 4-Cyanoindole Nucleosides, 4-Cyanoindole-2'-Deoxyribonucleoside-5'-Triphosphate (4CIN-TP), and Enzymatic Incorporation of 4CIN-TP into DNA [0.03%]
4-氰基吲哚核苷，4-氰基吲哚-2'-脱氧核糖三磷酸（4CIN-TP）以及DNA中的4CIN-TP酶促掺入反应的合成
Kellan T Passow,Nicole M Antczak,Shana J Sturla et al.
Kellan T Passow et al.
4-Cyanoindole-2'-deoxyribonucleoside (4CIN) is a fluorescent isomorphic nucleoside analogue with superior spectroscopic properties in terms of Stokes shift and quantum yield in comparison to the widely utilized isomorphic nucleoside analogu...
Copper-Catalyzed Huisgen 1,3-Dipolar Cycloaddition Tailored for Phosphorothioate Oligonucleotides [0.03%]
适用于磷硫酰 oligonucleotide 的铜催化的 Huisgen 1,3-偶极环加成反应
Malgorzata Honcharenko,Dmytro Honcharenko,Roger Stromberg
Malgorzata Honcharenko
An efficient method for attachment of a variety of reporter groups to oligonucleotides (ONs) is copper (I) [Cu(I)]-catalyzed Huisgen azide-alkyne 1,3-dipolar cycloaddition ("click reaction"). However, in the case of ONs with phosphorothioat...