Guillaume Clavé,Jean-Jacques Vasseur,Michael Smietana
Guillaume Clavé
This article contains detailed synthetic procedures for the implementation of the sulfo-click reaction to nucleoside derivatives. First, 3'-O-TBDMS-protected nucleosides are converted to their corresponding 4'-thioacid derivatives in three ...
Controlling Gene-Silencing with Azobenzene-Containing siRNAs (siRNAzos) [0.03%]
含偶氮苯的siRNA（siRNAzo）实现时空特异的基因干扰
Matthew L Hammill,Golam Islam,Jean-Paul Desaulniers
Matthew L Hammill
This article contains the detailed biophysical characterization, biological testing, and photo-switching protocols of azobenzene containing siRNAs (siRNAzos), which have photoswitchable properties that can be controlled with light. First, t...
Tandem Homometallic or Multimetallic Catalysis for Assembly of Base-Modified Nucleosides [0.03%]
串联同金属或多元金属催化碱基修饰核苷的组装
Harshita Shet,Shatrughn Bhilare,Yogesh S Sanghvi et al.
Harshita Shet et al.
Tandem catalysis has been at the forefront of synthesis in the past decade due to the reduction in the number of steps and purification needed for the synthesis of commercially relevant molecules. With the right combination of catalyst syst...
Efficient Synthesis of Trifluoromethylated Purine Ribonucleosides and Ribonucleotides [0.03%]
三氟甲基化嘌呤核糖核苷和核糖核苷酸的高效合成
Mikolaj Chrominski,Joanna Kowalska,Jacek Jemielity
Mikolaj Chrominski
The protocols presented in this article describe highly detailed synthesis of trifluoromethylated purine nucleotides and nucleosides (G and A). The procedure involves trifluoromethylation of properly protected (acetylated) nucleosides, foll...
NMR Spectroscopy of Large Functional RNAs: From Sample Preparation to Low-Gamma Detection [0.03%]
大型功能性RNA的核磁共振光谱：从样品制备到低γ检测
Robbin Schnieders,Bozana Knezic,Heidi Zetzsche et al.
Robbin Schnieders et al.
NMR spectroscopy is a potent method for the structural and biophysical characterization of RNAs. The application of NMR spectroscopy is restricted in RNA size and most often requires isotope-labeled or even selectively labeled RNAs. Additio...
Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis [0.03%]
使用凝胶电泳对DNA纳米结构进行核酸酶降解分析
Arun Richard Chandrasekaran,Ken Halvorsen
Arun Richard Chandrasekaran
Custom-built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such ap...
Synthesis of 5-Cyanomethyluridine (cnm5 U) and 5-Cyanouridine (cn5 U) Phosphoramidites and Their Incorporation into RNA Oligonucleotides [0.03%]
5-氰甲基尿苷(cnm5 U)和5-氰尿苷(cn5 U)的磷酰胺酯合成及其在RNA寡核苷酸中的应用
Song Mao,Hsu-Chun Tsai,Jia Sheng
Song Mao
This article contains detailed synthetic protocols for preparation of 5-cyanomethyluridine (cnm5 U) and 5-cyanouridine (cn5 U) phosphoramidites. The synthesis of the cnm5 U phosphoramidite building block starts with commercially available 5...
Detection and Quantification of RNA Phosphorothioate Modifications Using Mass Spectrometry [0.03%]
利用质谱法检测和定量RNA硫磷酸酯化修饰
Ying Wu,Ya Ying Zheng,Qishan Lin et al.
Ying Wu et al.
This article describes a protocol for detecting and quantifying RNA phosphorothioate modifications in cellular RNA samples. Starting from solid-phase synthesis of phosphorothioate RNA dinucleotides, followed by purification with reversed-ph...
Solid-Phase Synthesis of RNA 5'-Azides and Their Application for Labeling, Ligation, and Cyclization Via Click Chemistry [0.03%]
固相合成RNA 5'-叠氮及其通过点击化学的标记、连接和环化反应
Marcin Warminski,Joanna Kowalska,Jacek Jemielity
Marcin Warminski
RNAs with 5' functional groups have been gaining interest as molecular probes and reporter molecules. Copper-catalyzed azide-alkyne cycloaddition is one of the most straightforward methods to access such molecules; however, RNA functionaliz...
Dim and Dmoc Protecting Groups for Oligodeoxynucleotide Synthesis [0.03%]
暗保护基和 Democr 保护基在寡脱氧核糖核酸合成中的应用
Shiyue Fang,Dhananjani Eriyagama,Yinan Yuan et al.
Shiyue Fang et al.
This protocol provides details for the preparation of nucleoside phosphoramidites with 1,3-dithian-2-yl-methyl (Dim) and 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) as protecting groups, and a linker with Dmoc as the cleavable function, then us...