Anaïs Depaix,Suzanne Peyrottes,Béatrice Roy
Anaïs Depaix
This unit describes a one-pot, two step synthesis of ribonucleoside 5'-di- and 5'-triphosphates, as well as their purification. The first step of the synthesis involves the activation of an unprotected ribonucleoside 5'-monophosphate with 2...
Stereoselective Synthesis of 4'-Selenonucleosides via the Seleno-Michael Reaction [0.03%]
含硒核糖核酸碱基的立体选择性合成
Pramod K Sahu,Dnyandev B Jarhad,Gyudong Kim et al.
Pramod K Sahu et al.
5'-Homo-4'-selenonucleosides, a class of next-generation nucleosides, are synthesized from D-ribose via a 4-selenosugar intermediate. The key step in synthesizing this intermediate is a seleno-Michael reaction. 5'-Homo-4'-selenouridine and ...
A High-Throughput Process for the Solid-Phase Purification of Synthetic DNA Sequences [0.03%]
一种合成DNA序列固相纯化的高通量工艺流程
Andrzej Grajkowski,Jacek Cieślak,Serge L Beaucage
Andrzej Grajkowski
An efficient process for the purification of synthetic phosphorothioate and native DNA sequences is presented. The process is based on the use of an aminopropylated silica gel support functionalized with aminooxyalkyl functions to enable ca...
Jon D Moulton
Jon D Moulton
Morpholino oligonucleotides are stable, uncharged, water-soluble molecules used to block complementary sequences of RNA, preventing processing, read-through, or protein binding at those sites. Morpholinos are typically used to block transla...
2-Pyridinyl Thermolabile Groups as General Protectants for Hydroxyl, Phosphate, and Carboxyl Functions [0.03%]
2-吡啶基热不稳定的保护基在羟基、磷酸盐和羧酸中的应用
Jolanta Brzezinska,Agnieszka Witkowska,Tomasz P Kaczyński et al.
Jolanta Brzezinska et al.
Application of 2-pyridinyl thermolabile protecting groups (2-PyTPGs) for protection of hydroxyl, phosphate, and carboxyl functions is presented in this unit. Their characteristic feature is a unique removal process following the intramolecu...
Rui Wang,Phensinee Haruehanroengra,Jia Sheng
Rui Wang
This unit describes the chemical synthesis of the S-geranyl-2-thiouridine (ges2 U) phosphoramidite and its incorporation into RNA oligonucleotides through solid-phase synthesis. Starting from the 2-thiouracil nucleobase and the protected ri...
Rafael Del Villar-Guerra,Robert D Gray,Jonathan B Chaires
Rafael Del Villar-Guerra
Circular dichroism (CD) is a phenomenon that arises from the differential absorption of left- and right-handed circularly polarized light, and may be seen with optically active molecules. CD spectroscopy provides useful spectral signatures ...
Facile Access to Bromonucleosides Using Sodium Monobromoisocyanurate (SMBI) [0.03%]
简便的溴化核苷合成方法：使用一 bromoisocyanurate钠盐(SMBI)
Jyotirmoy Maity,Smriti Srivastava,Yogesh S Sanghvi et al.
Jyotirmoy Maity et al.
Bromonucleosides constitute a significant class of molecules and are well known for their biological activity. 5-Bromouridine, 5-bromo-2'-deoxyuridine, 5-bromouridine-5'-triphosphate, and nucleotides containing 5-bromouridine have been test...
Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes [0.03%]
计算化学修饰RNA双链变形性的方法及其相对稳定性的估计
Yoshiaki Masaki,Mitsuo Sekine,Kohji Seio
Yoshiaki Masaki
Chemical modification of RNA duplexes alters their stability. We have attempted to develop a computational approach to estimate the thermal stability of chemically modified duplexes. These studies revealed that the deformability of chemical...
Gram-Scale Chemical Synthesis of Base-Modified Ribonucleoside-5'-O-Triphosphates [0.03%]
用于化学合成碱基修饰核苷三磷酸的克级制备法
Muthian Shanmugasundaram,Annamalai Senthilvelan,Anilkumar R Kore
Muthian Shanmugasundaram
This unit delineates a simple, reliable, straight-forward, general, and efficient chemical method for the synthesis of modified nucleoside-5'-O-triphosphates such as 5-methylcytidine-5'-O-triphosphate (5-Me-CTP), pseudouridine-5'-O-triphosp...