Azospirillum brasilense, a Beneficial Soil Bacterium: Isolation and Cultivation [0.03%]
有益土壤细菌芽孢杆菌的分离与培养
Gladys Alexandre
Gladys Alexandre
Bacteria of the genus Azospirillum comprise 15 species to date, with A. brasilense the best studied species in the genus. Azospirillum are soil bacteria able to promote the growth of plants from 113 species spanning 35 botanical families. T...
Matthieu J Miossec,Sandro L Valenzuela,Katterinne N Mendez et al.
Matthieu J Miossec et al.
As the field of microbiomics advances, the burden of computational work that scientists need to perform in order to extract biological insight has grown accordingly. Likewise, while human microbiome analyses are increasingly shifting toward...
Dhritiman Samanta,Stacey D Gilk
Dhritiman Samanta
Coxiella burnetii is the causative agent of human Q fever, a zoonotic disease that can cause a debilitating, flu-like illness in acute cases, or a life-threatening endocarditis in chronic patients. An obligate intracellular bacterial pathog...
Subuhi Sherwani,Mohammed Chowdhury,Joachim J Bugert
Subuhi Sherwani
Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and is mostly self-limiting. In contrast to orthopoxviruses, MCV infections ...
Azospirillum brasilense: Laboratory Maintenance and Genetic Manipulation [0.03%]
巴西红圆褐固氮菌的实验室保藏及遗传操作
Jessica Gullett,Lindsey O&#x;Neal,Tanmoy Mukherjee et al.
Jessica Gullett et al.
Bacteria of the genus Azospirillum, including the most comprehensively studied Azospirillum brasilense, are non-pathogenic soil bacteria that promote the growth of diverse plants, making them an attractive model to understand non-symbiotic,...
Leptospira: Molecular Detection of Pathogenic Species in Natural Sources [0.03%]
通过分子检测方法鉴定自然水源中的致病性钩端螺旋体种属
Brittney Beigel,Ashutosh Verma
Brittney Beigel
This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) for...
Greg Harris,Rhonda KuoLee,H Howard Xu et al.
Greg Harris et al.
This unit describes basic protocols for infecting mice through intranasal and intraperitoneal routes with Acinetobacter baumannii to induce associated pneumonia and sepsis, the two most common manifestations of clinical infections with this...
Benjamin Allen,Meghan Drake,Nomi Harris et al.
Benjamin Allen et al.
The DOE Systems Biology Knowledgebase (KBase, http://kbase.us/) is an open-access bioinformatics software and data platform for analyzing plants, microbes, and their communities. KBase enables scientists to create, execute, collaborate on, ...
Mattie C Pawlowic,Sumiti Vinayak,Adam Sateriale et al.
Mattie C Pawlowic et al.
The apicomplexan parasite Cryptosporidium is a leading cause of diarrheal disease and an important contributor to overall global child mortality. We currently lack effective treatment and immune prophylaxis. Recent advances now permit genet...
Quantitative Dextran Trafficking to the Coxiella burnetii Parasitophorous Vacuole [0.03%]
定量测定地高辛转移到库克斯体寄生虫囊泡中的过程
Seth Winfree,Stacey D Gilk
Seth Winfree
The gram-negative bacterium Coxiella burnetii causes human Q fever, a disease characterized by a debilitating flu-like illness in acute cases and endocarditis in chronic patients. An obligate intracellular pathogen, Coxiella burnetii surviv...