MaRyka R Smith,Erin E Schirtzinger,William C Wilson et al.
MaRyka R Smith et al.
Rift Valley fever virus (RVFV) is an arthropod-borne, zoonotic disease endemic to sub-Saharan Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever have had up to 100% mortality rates in fetal and neonatal sheep. Upon infection o...
George D Ashton,Paul S Dyer
George D Ashton
Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was re...
Negative-Stain Transmission Electron Microscopy of Molecular Complexes for Image Analysis by 2D Class Averaging [0.03%]
通过二维同源平均法进行的分子复合物负染透射电子显微镜成像分析
John R Gallagher,Alexander J Kim,Neetu M Gulati et al.
John R Gallagher et al.
Negative-stain transmission electron microscopy (EM) is a technique that has provided nanometer resolution images of macromolecules for about 60 years. Developments in cryo-EM image processing have maximized the information gained from aver...
Can Zhao,Marcin G Fraczek,Lauren Dineen et al.
Can Zhao et al.
Aspergillus fumigatus is a human pathogen and the principal etiologic agent of invasive and chronic aspergillosis leading to several hundreds of thousands of deaths every year. Very few antifungals are available to treat infections caused b...
Florian A Thesseling,Peter W Bircham,Stijn Mertens et al.
Florian A Thesseling et al.
Beer would not exist without microbes. During fermentation, yeast cells convert cereal-derived sugars into ethanol and CO2 . Yeast also produces a wide array of aroma compounds that influence beer taste and aroma. The complex interaction be...
Fast and Reliable PCR Amplification from Aspergillus fumigatus Spore Suspension Without Traditional DNA Extraction [0.03%]
无需传统DNA提取的快速可靠的烟曲霉孢子悬液PCR扩增方法
Marcin G Fraczek,Can Zhao,Lauren Dineen et al.
Marcin G Fraczek et al.
Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for sing...
Neetu M Gulati,Udana Torian,John R Gallagher et al.
Neetu M Gulati et al.
Immunoelectron microscopy is a powerful technique for identifying viral antigens and determining their structural localization and organization within vaccines and viruses. While traditional negative staining transmission electron microscop...
Iván Pastor-Fernández,Elaine Pegg,Sarah E Macdonald et al.
Iván Pastor-Fernández et al.
Eimeria is a genus of apicomplexan parasites that contains a large number of species, most of which are absolutely host-specific. Seven species have been recognized to infect chickens. Infection of susceptible chickens results in an intesti...
Andrew L Chang,Camaron R Hole,Tamara L Doering
Andrew L Chang
Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis, which kills 200,000 individuals worldwide each year. It is ubiquitous in the environment and is first inhaled into the lungs of the host, where it ...
An In Vitro Brain Endothelial Model for Studies of Cryptococcal Transmigration into the Central Nervous System [0.03%]
用于研究隐球菌迁移到中枢神经系统的体外脑内皮模型
Felipe H Santiago-Tirado,Robyn S Klein,Tamara L Doering
Felipe H Santiago-Tirado
Cryptococcus neoformans is an environmental yeast found worldwide that causes lethal brain infections, particularly in immunocompromised hosts. In 2016, there were 280,000 cases of cryptococcal meningitis in the HIV+ population, two-thirds ...