Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 [0.03%]
两种详细的冠状病毒感染滴度测定平板法protocols
Emelissa J Mendoza,Kathy Manguiat,Heidi Wood et al.
Emelissa J Mendoza et al.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal agent of COronaVIrus Disease-19 (COVID-19), an atypical pneumonia-like syndrome that emerged in December 2019. While SARS-CoV-2 titers can be mea...
Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes [0.03%]
人乳头瘤伪病毒的生产和对原代人类角质形成细胞的感染
Samuel S Porter,Alison A McBride
Samuel S Porter
This protocol describes the production of human papillomavirus (HPV)-derived quasiviruses. Quasiviruses are infectious particles that are produced in 293TT packaging cells and contain a complete viral genome. We describe methods for infecti...
SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup [0.03%]
SARS-CoV-2血清转化的详细检测方法、抗原制备和试验设置方案
Daniel Stadlbauer,Fatima Amanat,Veronika Chromikova et al.
Daniel Stadlbauer et al.
In late 2019, cases of atypical pneumonia were detected in China. The etiological agent was quickly identified as a betacoronavirus (named SARS-CoV-2), which has since caused a pandemic. Several methods allowing for the specific detection o...
Using Direct RNA Nanopore Sequencing to Deconvolute Viral Transcriptomes [0.03%]
利用直接RNA纳米孔测序解卷病毒转录组
Daniel P Depledge,Angus C Wilson
Daniel P Depledge
The genomes of DNA viruses encode deceptively complex transcriptomes evolved to maximize coding potential within the confines of a relatively small genome. Defining the full range of viral RNAs produced during an infection is key to underst...
Aseptic Technique [0.03%]
无菌技术
Tomasz Bykowski,Brian Stevenson
Tomasz Bykowski
This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as "aseptic technique." Two major strategies for aseptic work are described: using a Bunsen burner and usi...
Alex Andrianopoulos
Alex Andrianopoulos
Talaromyces marneffei is an important opportunistic human pathogen endemic to Southeast Asia. It is one of a number of pathogenic fungi that exhibits thermally controlled dimorphism. At 25°C, T. marneffei grows in a multicellular, filament...
Step-by-Step Pipeline for the Ecological Analysis of Endophytic Fungi using ITS nrDNA Data [0.03%]
基于ITS核糖 DNA 数据的内生真菌生态分析的逐步流程管道
Maripaz Montero-Vargas,Efraín Escudero-Leyva,Stefani Díaz-Valerio et al.
Maripaz Montero-Vargas et al.
The nuclear ribosomal DNA internal transcribed spacer (ITS) is accepted as the genetic marker or barcode of choice for the identification of fungal samples. Here, we present a protocol to analyze fungal ITS data, from quality preprocessing ...
Deconstructing and Reconstructing Cheese Rind Microbiomes for Experiments in Microbial Ecology and Evolution [0.03%]
奶酪皮微生物群的拆解与重建——用于实验的微生物生态和进化研究
Casey M Cosetta,Benjamin E Wolfe
Casey M Cosetta
Cheese rind microbiomes are useful model systems for identifying the mechanisms that control microbiome diversity. Here, we describe the methods we have optimized to first deconstruct in situ cheese rind microbiome diversity and then recons...
Shelley M Payne
Shelley M Payne
Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and...
Brianna Ball,Jennifer Geddes-McAlister
Brianna Ball
Cryptococcus neoformans is an opportunistic human fungal pathogen commonly associated with infection in immunocompromised individuals (e.g., patients with HIV/AIDS). Important virulence determinants include the production of a polysaccharid...