Claus Sternberg,Tim Tolker-Nielsen
Claus Sternberg
The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. ...
Safe use of radioisotopes [0.03%]
放射性同位素的安全使用
Jill Meisenhelder,Kentaro Semba
Jill Meisenhelder
The use of radioisotopes to label specific molecules in a defined way has greatly furthered the discovery and dissection of biochemical pathways. The development of methods to synthesize such tagged biological compounds inexpensively on an ...
George Lunn,Gretchen Lawler
George Lunn
This appendix provides protocols for some commonly used disposal and decontamination procedures along with analytical techniques that are used to verify that reagents have been decontaminated. Some of the specific reagents covered are diami...
Biosafety practices associated with potential agents of biocrime and biowarfare [0.03%]
与生物犯罪和生物 warfare潜在因素相关的生物安全实践
LouAnn C Burnett
LouAnn C Burnett
Conducting research in a manner that guards against theft and intentional misuse of biological materials requires a process of hazard identification and risk assessment to most effectively identify and implement a risk management plan. Proc...
Brian Stevenson
Brian Stevenson
This appendix provides general techniques, equipment, and media used for the growth of many commonly encountered bacteria. For specific growth conditions, readers should refer to units regarding the laboratory maintenance of the organism of...
Helena Harlin,Thomas F Gajewski
Helena Harlin
Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphoryla...
This appendix provides commonly used abbreviations for many terms used in this manual.
Yao-Hui Sun,Andreas B den Hartigh,Renée M Tsolis et al.
Yao-Hui Sun et al.
This unit provides protocols for growth of Brucella abortus on solid or in liquid media and for long-term storage of laboratory stocks. Two issues affecting the culture and storage of isolates of this slow-growing bacterium are emphasized: ...
Jason A Carlyon
Jason A Carlyon
Anaplasma phagocytophilum is the etiologic agent of human granulocytic anaplasmosis (formerly human granulocytic ehrlichiosis), an emerging and potentially deadly disease in the United States, Europe, and Asia. A. phagocytophilum is an obli...
Edward D Salmon,Kate von Lackum,Julie C Canman
Edward D Salmon
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contras...