Genetic Manipulation of Corynebacterium diphtheriae and Other Corynebacterium Species [0.03%]
白喉棒状杆菌及其他棒状杆菌的基因操作
Chungyu Chang,Minh Tan Nguyen,Hung Ton-That
Chungyu Chang
This article describes several established approaches for genetic manipulation of Corynebacterium diphtheriae, the causative agent of diphtheria that is known to have provided key evidence for Koch's postulates on the germ theory. First, it...
A Low-Cost Tebuconazole-Based Screening Test for Azole-Resistant Aspergillus fumigatus [0.03%]
基于Tebuconazole的低费用筛查试验用于检测抗唑类曲霉菌
Amelie P Brackin,Jennifer M G Shelton,Alireza Abdolrasouli et al.
Amelie P Brackin et al.
The global emergence of azole resistance in Aspergillus fumigatus is resulting in health and food security concerns. Rapid diagnostics and environmental surveillance methods are key to understanding the distribution and prevalence of azole ...
Alaa A Abdelmageed,Maureen C Ferran
Alaa A Abdelmageed
Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative-sense single-stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine ...
Corynebacterium diphtheriae Virulence Analyses Using a Caenorhabditis elegans Model [0.03%]
模式生物Caenorhabditis elegans感染模型在白喉棒状杆菌致病性研究中的应用
Yi-Wei Chen,Hung Ton-That
Yi-Wei Chen
Corynebacterium diphtheriae is the leading cause of pharyngeal diphtheria, a respiratory disease characterized by formation of a pseudomembrane at the site of infection. Although outbreaks of C. diphtheriae infections are rare nowadays, the...
Human Bocavirus 1 Infection of Well-Differentiated Human Airway Epithelium [0.03%]
人博卡病毒1型感染人体气道上皮细胞层模型
Ziying Yan,Xuefeng Deng,Jianming Qiu
Ziying Yan
Human bocavirus 1 (HBoV1) is a small DNA virus that belongs to the Bocaparvovirus genus of the Parvoviridae family. HBoV1 is a common respiratory pathogen that causes mild to life-threatening acute respiratory tract infections in children a...
Jane M Jayakumar,Orr H Shapiro,Salvador Almagro-Moreno
Jane M Jayakumar
Vibrio vulnificus, an emergent human pathogen, causes fulminant septicemia with a mortality rate of over 50%. Unlike for other pathogenic Vibrio species, the factors to conclusively indicate the virulence potential of V. vulnificus strains ...
An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening [0.03%]
一种用于新冠病毒血清学和药物筛选的体外微中和实验方法
Fatima Amanat,Kris M White,Lisa Miorin et al.
Fatima Amanat et al.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activ...
Emily A Peluso,Matthew Scheible,Hung Ton-That et al.
Emily A Peluso et al.
Considered a commensal, the Gram-negative anaerobe Fusobacterium nucleatum is a key member of the oral microbiome due to its wide range of interactions with many oral microbes. While the periodontal pathogenic properties of this organism ha...
Giardia lamblia: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models [0.03%]
兰伯氏贾第鞭毛虫：实验室培养、生活周期诱导和感染小鼠模型
Marc Y Fink,Danielle Shapiro,Steven M Singer
Marc Y Fink
Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. In...
Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays [0.03%]
维氏弧菌：实验室培养、保存及常见表型检测方法
David G Christensen,Karen L Visick
David G Christensen
Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. ...