Makayla R P Dean,Elizabeth M Duncan
Makayla R P Dean
Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians...
Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chromosome [0.03%]
使用细菌人工染色体生成重组SARS-CoV-2病毒
Kevin Chiem,Chengjin Ye,Luis Martinez-Sobrido
Kevin Chiem
SARS-CoV-2, the causative agent of COVID-19, has been responsible for a million deaths worldwide as of September 2020. At the time of this writing, there are no available US FDA-approved therapeutics for the treatment of SARS-CoV-2 infectio...
Kali R Iyer,Nicole Robbins,Leah E Cowen
Kali R Iyer
A technique to assess the ability of distinct Candida strains to efflux substrates, as well as to compare the effectiveness of efflux inhibitors, is important for analysis of antifungal drug resistance mechanisms and the mode of action of a...
Samuel Plante,Christian R Landry
Samuel Plante
Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes t...
High-Yield Purification of Giardia intestinalis Cysts from Fecal Samples [0.03%]
来自粪便样本的Giardia intestinalis包囊的高纯度净化过程
Paul Ogbuigwe,Anthony B Pita,Matthew A Knox et al.
Paul Ogbuigwe et al.
Giardia is an enteric protozoan parasite that causes gastroenteritis in all classes of vertebrates. It is ranked among the leading causes of death in children under 5 years of age. Giardiasis affects approximately 280 million people worldwi...
Ixodid Tick Dissection and Tick Ex Vivo Organ Cultures for Tick-Borne Virus Research [0.03%]
用于蜱传病毒研究的蜱解剖和蜱离体器官培养技术
Jeffrey M Grabowski,Ryan Kissinger
Jeffrey M Grabowski
Tick-borne viruses cause thousands of cases of disease worldwide every year. Specific countermeasures to many tick-borne viruses are not commercially available. Very little is known regarding tick-virus interactions and increasing this know...
Experimental Evolution of Antifungal Resistance in Cryptococcus neoformans [0.03%]
新型隐球菌抗真菌耐药性的实验进化研究
Arianne Bermas,Jennifer Geddes-McAlister
Arianne Bermas
Cryptococcus neoformans, an opportunistic yeast-like fungal pathogen, has demonstrated resistance to all major classes of antifungals used to treat cryptococcal meningitis. However, combatting this fungal disease is an ongoing challenge amo...
David G Christensen,Jovanka Tepavčević,Karen L Visick
David G Christensen
Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species. The bacterium has been used as a model organism to study symbiosis in the context of its association with its host, the Hawaiian bobtail squid Euprymna scolop...
A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis [0.03%]
一个用于研究白色念珠菌病原性的简单的线虫感染模型
Grace H Kim,Sierra Rosiana,Natalia V Kirienko et al.
Grace H Kim et al.
Candida albicans is an opportunistic fungal pathogen and a model organism to study fungal pathogenesis. It exists as a harmless commensal organism and member of the healthy human microbiome, but can cause life-threatening mucosal and system...
Proper Care and Feeding of Coccidioides: A Laboratorian's Guide to Cultivating the Dimorphic Stages of C. immitis and C. posadasii [0.03%]
球孢子菌的正确护理和喂养：实验室人员培养C. immitis 和 C. posadasii双相阶段的指南
Heather L Mead,Marley C Caballero Van Dyke,Bridget M Barker
Heather L Mead
Coccidioidomycosis ("Valley fever") is caused by Coccidioides immitis and C. posadasii. These fungi are thermally dimorphic, cycling between mycelia and arthroconidia in the environment and converting into spherules and endospores within a ...