Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi [0.03%]
用于多种细菌的可移动型CRISPRi基因敲低系统
Amy B Banta,Ryan D Ward,Jennifer S Tran et al.
Amy B Banta et al.
Facile bacterial genome sequencing has unlocked a veritable treasure trove of novel genes awaiting functional exploration. To make the most of this opportunity requires powerful genetic tools that can target all genes in diverse bacteria. C...
Gregory C Kujoth,Thomas D Sullivan,Bruce S Klein
Gregory C Kujoth
Dimorphic fungi in the genera Blastomyces, Histoplasma, Coccidioides, and Paracoccidioides are important human pathogens that affect human health in many countries throughout the world. Understanding the biology of these fungi is important ...
Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors [0.03%]
副溶血性弧菌：生长、遗传操作和毒力因子分析的基本技术
Suneeta Chimalapati,Alexander E Lafrance,Luming Chen et al.
Suneeta Chimalapati et al.
Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires proce...
3D Oral and Cervical Tissue Models for Studying Papillomavirus Host-Pathogen Interactions [0.03%]
用于研究乳头瘤病毒宿主病原体相互作用的三维口腔和宫颈组织模型
Robert Jackson,Jason D Maarsingh,Melissa M Herbst-Kralovetz et al.
Robert Jackson et al.
Human papillomavirus (HPV) infection occurs in differentiating epithelial tissues. Cancers caused by high-risk types (e.g., HPV16 and HPV18) typically occur at oropharyngeal and anogenital anatomical sites. The HPV life cycle is differentia...
Dissecting the Biology of the Fungal Wheat Pathogen Zymoseptoria tritici: A Laboratory Workflow [0.03%]
解剖小麦真菌病原Zymoseptoria tritici的生物学：实验室工作流程
Wagner C Fagundes,Janine Haueisen,Eva H Stukenbrock
Wagner C Fagundes
The fungus Zymoseptoria tritici is one of the most devastating pathogens of wheat. Aside from its importance as a disease-causing agent, this species has emerged as a powerful model system for evolutionary genetic studies of crop-infecting ...
Counter-Selection Method for Markerless Allelic Exchange in Bordetella bronchiseptica Based on sacB Gene From Bacillus subtilis [0.03%]
基于枯草芽孢杆菌sacB基因的副嗜血杆菌非标记等位基因交换的反向选择方法
Nicolás Ambrosis,Julieta Fernández,Federico Sisti
Nicolás Ambrosis
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory tract infections. It is a natural pathogen of a wide variety of mammals, including some used as laboratory models. This makes B. bronchiseptica an ideal organism...
A Mouse Model of Sublethal Leptospirosis: Protocols for Infection with Leptospira Through Natural Transmission Routes, for Monitoring Clinical and Molecular Scores of Disease, and for Evaluation of the Host Immune Response [0.03%]
亚致死性钩端螺旋体病小鼠模型：通过自然传播途径感染、监测临床和分子疾病评分以及评估宿主免疫反应的方案
Nisha Nair,Maria Gomes-Solecki
Nisha Nair
Leptospirosis is a zoonotic disease caused by pathogenic Leptospira species that are maintained in sylvatic and domestic environments by transmission among rodents and other carriers. Humans become infected after contact of breached skin or...
Monitoring Inflammasome Priming and Activation in Response to Candida albicans [0.03%]
监测白色念珠菌诱导的炎性小体启动和激活
Darian J Santana,Faith M Anderson,Teresa R O&#x;Meara
Darian J Santana
Candida albicans is a common mucosal colonizer, as well as a cause of lethal invasive fungal infections. The major predisposing factor for invasive fungal disease is a compromised immune system. One component of the host immune response to ...
Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA [0.03%]
假结核耶尔森菌：培养，储存以及引入DNA的方法
Robert K Davidson,Kimberly M Davis
Robert K Davidson
Yersinia pseudotuberculosis has been studied for many decades, and research on this microbe has taught us a great deal about host-pathogen interactions, bacterial manipulation of host cells, virulence factors, and the evolution of pathogens...
An Exonuclease V-qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and Tissues [0.03%]
用于分析细胞系和组织中人乳头瘤病毒16基因组状态的核酸外切酶V-qPCR测定方法
Julia E Myers,Katarzyna Zwolinska,Martin J Sapp et al.
Julia E Myers et al.
Integration of the human papillomavirus (HPV) genome into host cell chromosomes has been observed in a majority of HPV-positive cervical cancers and a subset of oral HPV-associated cancers. HPV integration also occurs in long-term cell cult...