A High-Throughput (HTS) Assay for Enzyme Reaction Phenotyping in Human Recombinant P450 Enzymes Using LC-MS/MS [0.03%]
一种基于LC-MS/MS的人重组P450酶反应表型的高通量（HTS）分析方法
Xiaofeng Li,Tom Suhar,Lateca Glass et al.
Xiaofeng Li et al.
Enzyme reaction phenotyping is employed extensively during the early stages of drug discovery to identify the enzymes responsible for the metabolism of new chemical entities (NCEs). Early identification of metabolic pathways facilitates pre...
Ross Jeggo,Fei-Yue Zhao,David Spanswick
Ross Jeggo
Understanding the physiology, pharmacology, and plasticity associated with synaptic function is a key goal of neuroscience research and is fundamental to identifying the processes involved in the development and manifestation of neurologica...
Alan D Wickenden
Alan D Wickenden
This unit provides an overview of the principal electrophysiological techniques commonly used for the study of ionic currents and the ion channels that mediate them. These techniques include electroencephalograms (EEGs), electrocardiograms ...
Brian L Furman
Brian L Furman
Streptozotocin (STZ) is an antibiotic that produces pancreatic islet β-cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZ-induced ...
Models of Inflammation: Carrageenan- or Complete Freund's Adjuvant (CFA)-Induced Edema and Hypersensitivity in the Rat [0.03%]
炎症模型：用κ- carrageenan或完全弗氏佐剂(CFA)致大鼠足肿胀和迟发型超敏反应模型
Kenneth E McCarson
Kenneth E McCarson
Animal models of inflammation are used to assess the production of inflammatory mediators at sites of inflammation, the processing of pain sensation at CNS sites, the anti-inflammatory properties of agents such as nonsteroidal anti-inflamma...
Using Bioluminescence Resonance Energy Transfer (BRET) to Characterize Agonist-Induced Arrestin Recruitment to Modified and Unmodified G Protein-Coupled Receptors [0.03%]
采用生物发光共振能量转移(BRET)技术研究配体诱导的G蛋白偶联受体相互作用 Arrestin募集作用的研究
Prashant Donthamsetti,Jose Rafael Quejada,Jonathan A Javitch et al.
Prashant Donthamsetti et al.
G protein-coupled receptors (GPCRs) represent ∼25% of current drug targets. Ligand binding to these receptors activates G proteins and arrestins, which are involved in differential signaling pathways. Because functionally selective or bias...
Overview of Genetically Engineered Mouse Models of Breast Cancer Used in Translational Biology and Drug Development [0.03%]
用于转化生物学和药物开发的乳腺癌转基因小鼠模型概览
Kirsty R Greenow,Matthew J Smalley
Kirsty R Greenow
Breast cancer is a heterogeneous condition with no single standard of treatment and no definitive method for determining whether a tumor will respond to therapy. The development of murine models that faithfully mimic specific human breast c...
Overview of KRAS-Driven Genetically Engineered Mouse Models of Non-Small Cell Lung Cancer [0.03%]
KRAS驱动的非小细胞肺癌基因工程小鼠模型概述
Clare Sheridan,Julian Downward
Clare Sheridan
KRAS, the most frequently mutated oncogene in non-small cell lung cancer, has been utilized extensively to model human lung adenocarcinomas. The results from such studies have enhanced considerably an understanding of the relationship betwe...
Arrestin-3-Dependent Activation of c-Jun N-Terminal Kinases (JNKs) [0.03%]
由 Arrestin-3 介导的 c-Jun氨基末端激酶(JNK)活化作用
Xuanzhi Zhan,Seunghyi Kook,Tamer S Kaoud et al.
Xuanzhi Zhan et al.
Only one out of four mammalian arrestin subtypes, arrestin-3, facilitates the activation of JNK family kinases. Here we describe two different protocols used for elucidating the mechanisms involved. One is based on reconstitution of signali...
Generation of human acute lymphoblastic leukemia xenografts for use in oncology drug discovery [0.03%]
人急性淋巴细胞白血病异种移植瘤的建立在肿瘤药物发现中的应用
Linda Holmfeldt,Charles G Mullighan
Linda Holmfeldt
The establishment of reproducible mouse models of acute lymphoblastic leukemia (ALL) is necessary to provide in vivo therapeutic test systems that recapitulate human ALL, and for amplification of limited amounts of primary tumor material. A...