Label-Free Dynamic Mass Redistribution and Bio-Impedance Methods for Drug Discovery [0.03%]
无标签动态质量重组和生物阻抗法在药物发现中的应用
Manuel Grundmann
Manuel Grundmann
Label-free biosensors are increasingly employed in drug discovery. Cell-based biosensors provide valuable insights into the biological consequences of exposing cells and tissues to chemical agents and the underlying molecular mechanisms ass...
Daniel J Holder,Michael J Marino
Daniel J Holder
Lack of reproducibility has been highlighted as a significant problem in biomedical research. The present unit is devoted to describing ways to help ensure that research findings can be replicated by others, with a focus on the design and e...
Assessment of Ex Vivo Transport Function in Isolated Rodent Brain Capillaries [0.03%]
大鼠和小鼠脑血管切片的离体转运功能评估研究
Gary N Y Chan,Ronald E Cannon
Gary N Y Chan
The blood-brain barrier plays an important role in neuroprotection; however, it can be a major obstacle for drug delivery to the brain. This barrier primarily resides in the brain capillaries and functions as an interface between the brain ...
Terry Kenakin
Terry Kenakin
Seven-transmembrane receptors (7TMRs or GPCRs [G protein-coupled receptors]) are nature's prototypic allosteric proteins in that they mediate the interaction between ligand binding to the receptor and the receptor interacting with another c...
Biomarkers-A General Review [0.03%]
生物标志物-综述
Jeffrey K Aronson,Robin E Ferner
Jeffrey K Aronson
A biomarker is a biological observation that substitutes for and ideally predicts a clinically relevant endpoint or intermediate outcome that is more difficult to observe. The use of clinical biomarkers is easier and less expensive than dir...
Functional Studies of Sodium Channels: From Target to Compound Identification [0.03%]
钠通道的功能研究：从靶点到化合物识别
Daniel Bertrand,Bruno Biton,Thomas Licher et al.
Daniel Bertrand et al.
Over the last six decades, voltage-gated sodium (Nav ) channels have attracted a great deal of scientific and pharmaceutical interest, driving fundamental advances in both biology and technology. The structure and physiological function of ...
Detection of Receptor Heteromerization Using In Situ Proximity Ligation Assay [0.03%]
利用原位邻近连接法检测受体异聚体形成
Ivone Gomes,Salvador Sierra,Lakshmi A Devi
Ivone Gomes
Although G protein-coupled receptor (GPCR) heteromerization has been extensively demonstrated in vitro using heterologous cells that overexpress epitope-tagged receptors, their presence in endogenous systems is less well established. This i...
Stability of Drugs, Drug Candidates, and Metabolites in Blood and Plasma [0.03%]
药物、候选药物及其代谢产物在血液和血浆中的稳定性分析
Gregory A Reed
Gregory A Reed
Determination of drug or drug metabolite concentrations in biological samples, particularly in serum or plasma, is fundamental to describing the relationships between administered dose, route of administration, and time after dose for achie...
Chris Towne,Kimberly R Thompson
Chris Towne
Optogenetics is a method that uses light to control cells in living tissue, typically neurons, that have been modified to express light-sensitive ion channels and pumps. The approach facilitates neuromodulation in brain preparations and fre...
Visualization of 5-HT Receptors Using Radioligand-Binding Autoradiography [0.03%]
放射配体结合自显影技术在五羟色胺受体可视化中的应用
Roser Cortés,M Teresa Vilaró,Guadalupe Mengod
Roser Cortés
Described in this unit are techniques to visualize the majority of serotonin (5-hydroxytryptamine, 5-HT) receptor subtypes in sections of frozen brain tissue using receptor autoradiography. Protocols for brain extraction and sectioning, rad...