Shaili Aggarwal,Ole V Mortensen
Shaili Aggarwal
The dopamine (DAT), serotonin (SERT), and norepinephrine (NET) transporters, which are collectively referred to as monoamine transporters (MATs), play significant roles in regulating the neuronal response to these neurotransmitters. MATs te...
Sujay Ramanathan,Botros B Shenoda,Seena K Ajit
Sujay Ramanathan
MicroRNA(miRNA)-mediated gene regulation underlies cellular processes, playing an important role in homeostasis and diseases. The expression and function of miRNAs are altered by various pharmacological agents, with differences in the endog...
In Vitro Assays for the Functional Characterization of the Dopamine Transporter (DAT) [0.03%]
用于多巴胺转运蛋白功能表征的体外试验
Shaili Aggarwal,Ole V Mortensen
Shaili Aggarwal
Detailed in this unit are protocols for studying the in vitro uptake of dopamine (DA) as a means for defining the functional characteristics of dopamine transporters. All assays are performed using commercially available cell lines that tra...
In Vitro Validation of miRNA-Mediated Gene Expression Linked to Drug Metabolism [0.03%]
体外验证与药物代谢相关的miRNA介导的基因表达
Botros B Shenoda,Sujay Ramanathan,Seena K Ajit
Botros B Shenoda
Pharmacogenomic approaches used to investigate how genes affect drug responses are critical for designing personalized therapies aimed at maximizing efficacy and minimizing adverse effects. Drug efficacy is often dependent on the sequence a...
Murine Retrovirally-Transduced Bone Marrow Engraftment Models of MLL-Fusion-Driven Acute Myelogenous Leukemias (AML) [0.03%]
MLL融合驱动的急性髓系白血病（AML）的小鼠 retroviral转导骨髓植入模型
Matthew C Stubbs,Andrei V Krivtsov
Matthew C Stubbs
MLL-rearranged leukemia represents approximately 5% to 10% of adult acute myelogenous leukemia (AML) and nearly half of all infant/pediatric acute leukemia cases. These leukemias have a poor prognosis, and there are no approved therapeutic ...
All-Optical Electrophysiology for Disease Modeling and Pharmacological Characterization of Neurons [0.03%]
用于疾病建模和神经元药理学特征表征的全光学电生理学技术
Christopher A Werley,Ted Brookings,Hansini Upadhyay et al.
Christopher A Werley et al.
A key challenge for establishing a phenotypic screen for neuronal excitability is measurement of membrane potential changes with high throughput and accuracy. Most approaches for probing excitability rely on low-throughput, invasive methods...
Development and Applications of Patient-Derived Xenograft Models in Humanized Mice for Oncology and Immune-Oncology Drug Discovery [0.03%]
人源化小鼠来源的患者类器官异种移植模型在肿瘤和免疫药物研发中的应用与发展
Bhavna Verma,Michael Ritchie,Maria Mancini
Bhavna Verma
With the recent approval of four novel immune oncology agents for the treatment of various cancers, the emerging power of this drug class has been substantiated. However, the full potential of such agents is yet to be realized, with only a ...
Rodent Test of Attention and Impulsivity: The 5-Choice Serial Reaction Time Task [0.03%]
注意力和冲动性啮齿类动物试验：五选连续反应时间任务
Guy A Higgins,Leo B Silenieks
Guy A Higgins
The 5-choice serial reaction time task (5-CSRTT) is employed extensively to measure attention in rodents. The assay involves animals trained to respond to a brief, unpredictable visual stimulus presented in one of five locations. The effect...
The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine [0.03%]
乌辛槽检测技术研究药物在人体肠道中的代谢与转运作用
Beatrice Kisser,Eva Mangelsen,Caroline Wingolf et al.
Beatrice Kisser et al.
The Ussing chamber is an old but still powerful technique originally designed to study the vectorial transport of ions through frog skin. This technique is also used to investigate the transport of chemical agents through the intestinal bar...
Overview of Transgenic Mouse Models of Myeloproliferative Neoplasms (MPNs) [0.03%]
关于髓系增生性肿瘤的小鼠转基因模型的综述
Andrew Dunbar,Abbas Nazir,Ross Levine
Andrew Dunbar
Myeloproliferative neoplasms (MPNs) are a class of hematologic diseases characterized by aberrant proliferation of one or more myeloid lineages and progressive bone marrow fibrosis. In 2005, seminal work by multiple groups identified the JA...