Utilizing Miniature Fluorescence Microscopy to Image Hippocampal Place Cell Ensemble Function in Thy1.GCaMP6f Transgenic Mice [0.03%]
利用微型荧光显微技术在Thy1.GCaMP6f转基因小鼠海马中成像位置细胞簇功能
Tim Indersmitten,Tamara Berdyyeva,Leah Aluisio et al.
Tim Indersmitten et al.
Imaging neuronal activity in awake behaving mice with miniature fluorescence microscopes requires the implementation of a variety of procedures. Surgeries are performed to gain access to the cell population of interest and to implant micros...
Quantitative Intracerebral Microdialysis Studies to Determine Unbound Extracellular Fluid Drug Concentrations in Discrete Areas of the Brain [0.03%]
脑内不同区域离体自由脑外液药物浓度的定量研究
Matthew R Durk
Matthew R Durk
Intracerebral microdialysis is a powerful technique for quantifying unbound brain extracellular fluid concentrations of drugs and drug candidates over time in live, conscious, freely moving animals. The results provide crucial information o...
Three Decades of Chloride Intracellular Channel Proteins: From Organelle to Organ Physiology [0.03%]
氯离子细胞内通道蛋白研究三十年：从细胞器到器官生理学
Shubha Gururaja Rao,Devasena Ponnalagu,Neel J Patel et al.
Shubha Gururaja Rao et al.
Intracellular organelles are membranous structures central for maintaining cellular physiology and the overall health of the cell. To maintain cellular function, intracellular organelles are required to tightly regulate their ionic homeosta...
Binding Assays for Bromodomain Proteins: Their Utility in Drug Discovery in Oncology and Inflammatory Disease [0.03%]
用于药物发现的溴结构域蛋白质检测方法及其在肿瘤和炎症性疾病中的应用价值研究
Nina I Zolotarjova,Richard Wynn
Nina I Zolotarjova
Bromodomains are protein domains that recognize acetylated lysine residues and are important for recruiting a large number of protein and multiprotein complexes to sites of lysine acetylation. They play an important role in chromatin biolog...
In Vitro Enzyme Assays for JmjC-Domain-Containing Lysine Histone Demethylases (JmjC-KDMs) [0.03%]
组蛋白赖氨酸去甲基化酶（JmjC-KDM）的体外酶活检测方法
Hanna Tarhonskaya,Anthony Tumber,Akane Kawamura et al.
Hanna Tarhonskaya et al.
Histone modifications, including lysine methylation marks on histone tails, modulate the accessibility of genes for transcription. Changes in histone tail methylation patterns can cause transcriptional activation or repression. The dynamic ...
Michael Williams
Michael Williams
Many results reported in the biomedical research literature cannot be independently reproduced, undermining the basic foundations of science. This overview is intended for researchers who are committed to improving the quality and integrity...
Overview of Genetically Engineered Mouse Models of Prostate Cancer and Their Applications in Drug Discovery [0.03%]
基因工程前列腺癌小鼠模型概览及其在药物发现中的应用
Gianluca Civenni,Giuseppina M Carbone,Carlo V Catapano
Gianluca Civenni
Prostate cancer (PCa) is the most common malignant visceral neoplasm in males in Western countries. Despite progress made in the early treatment of localized malignancies, there remains a need for therapies effective against advanced forms ...
Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers [0.03%]
脂质双层中的离子通道的重组及电特性的鉴定
Dan A Klaerke,Maria de Los Angeles Tejada,Vibeke Grøsfjeld Christensen et al.
Dan A Klaerke et al.
Detergent-solubilized purified ion channels can be reconstituted into lipid bilayers for electrophysiological analysis. Traditionally, ion channels were inserted into vesicles and subsequently fused with planar "black lipid membranes" forme...
Doodipala Samba Reddy,Xin Wu,Victoria M Golub et al.
Doodipala Samba Reddy et al.
Histone deacetylases (HDACs) represent a family of enzymes that are targets for epigenetic modulation of genomic activity and may be beneficial in the treatment of many diseases, including cancer and central nervous system disorders. In ani...
James A L Brown
James A L Brown
Acetylation is a core cellular process involved in maintaining genomic integrity, gene regulation, and metabolism. Histone acetyltransferases (HATs) are an enzyme family that regulates these processes by catalyzing the transfer of an acetyl...