Evaluation of Compound Activity in Primary Human Intestinal Organoids Using Gene Expression and Histology [0.03%]
基于基因表达和组织学的人源原肠类器官化合物活性评价
Ronald Marchelletta,Jingxue Yu,Clara Moon et al.
Ronald Marchelletta et al.
Human intestinal organoids have enabled performance of functional epithelial studies and modeling of human diseases of the intestine. This unit describes 1) a method to isolate and culture crypts from human intestinal tissue, 2) use of comb...
Translational Use of a Standardized Full Human Skin Organ Culture Model in Autoimmune Blistering Diseases [0.03%]
自身免疫性大疱病中标准化完整人体皮肤器官培养模型的转化应用
Imke A K Burmester,Shirin Emtenani,Jan-Gerrit Johns et al.
Imke A K Burmester et al.
The full human skin organ culture (HSOC) model is a standardized test system for evaluating pharmacological substances on human skin in vitro. The acantholysis associated with pemphigus vulgaris (PV), a severe and potentially life-threateni...
Preclinical Models of Alzheimer's Disease: Relevance and Translational Validity [0.03%]
阿尔茨海默病的疾病模型：相关性和转化有效性
Kevin Mullane,Michael Williams
Kevin Mullane
The only drugs currently approved for the treatment of Alzheimer's Disease (AD) are four acetylcholinesterase inhibitors and the NMDA antagonist memantine. Apart from these drugs, which have minimal to no clinical benefit, the 40-year searc...
Anika Kasprick,Katja Bieber,Ralf J Ludwig
Anika Kasprick
Pemphigoid diseases (PDs) are a group of autoimmune bullous diseases characterized and caused by autoantibodies targeting structural proteins of the skin and mucous membranes. Chronic inflammation, subepidermal blistering, and often scaring...
Ana S Leal,Karen T Liby
Ana S Leal
Chronic or repeated episodes of acute pancreatic inflammation, or pancreatitis, are risk factors for the development of pancreatic cancer. Pancreatic cancer is characterized by a strong fibro-inflammatory tumor microenvironment. In pancreat...
Modeling Chronic Graft Versus Host Disease in Mice Using Allogeneic Bone Marrow and Splenocyte Transfer [0.03%]
利用异基因骨髓和脾细胞移植在小鼠中构建慢性移植物抗宿主病模型
Mark A Schroeder,Kidist Ashami,Karl Staser
Mark A Schroeder
This unit describes a method for allogeneic bone marrow and splenocyte transfer for the modeling of chronic graft versus host disease (cGVHD) in mice. Preclinical models provide clinically relevant platforms for mechanistic and therapeutic ...
The Irwin Test and Functional Observational Battery (FOB) for Assessing the Effects of Compounds on Behavior, Physiology, and Safety Pharmacology in Rodents [0.03%]
伊文测试及行为学功能观测电池(FOB)在评估化合物对啮齿类动物的行为、生理和安全药理的影响方面的应用
Joanne R Mathiasen,Virginia C Moser
Joanne R Mathiasen
The modified Irwin procedure or functional observational battery (FOB) can be used to achieve several goals. New chemical entities (NCEs) can be behaviorally screened for nervous system effects at a variety of doses to identify potential th...
Yan Zhang
Yan Zhang
Transporters play important roles in absorption, distribution, metabolism, and elimination (ADME) processes, as well as drug pharmacokinetics (PK) and pharmacodynamics (PD). They are also important in maintaining the homeostasis of endogeno...
Caroline S Colley,Elizabeth England,John E Linley et al.
Caroline S Colley et al.
Ion channels play crucial roles in physiology by modulation of cellular functions that include electrical excitability, secretion, cell migration, and gene transcription. They are an important target class for drug discovery and have histor...
Protocols for Measuring Glutamate Uptake: Dose-Response and Kinetic Assays in In Vitro and Ex Vivo Systems [0.03%]
谷氨酸摄取测定的协议：体外和离体系统中的剂量反应和动力学测定法
Andréia C K Fontana
Andréia C K Fontana
This article presents detailed descriptions of procedures and troubleshooting tips for basic in vitro and ex vivo uptake assays for the functional characterization of glutamate transporters and the assessment of the effect of compounds that...