Automated Dynamic Clamp for Simulation of IK1 in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Real Time Using Patchliner Dynamite8 [0.03%]
使用Patchliner Dynamite8在实时中自动模拟人类诱导多能干细胞衍生心肌细胞中IK1的动态钳夹技术
Nadine Becker,András Horváth,Teun De Boer et al.
Nadine Becker et al.
Current in vitro assays typically poorly predict cardiac liability as they focus on single ion channels overexpressed in cell lines. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), on the other hand, provide a unique...
Assays for Modulators of Ryanodine Receptor (RyR)/Ca2+ Release Channel Activity for Drug Discovery for Skeletal Muscle and Heart Diseases [0.03%]
用于发现治疗肌病和心脏疾病药物的调控ryanodine受体(RyR)/Ca2+释放通道活性检定方法
Takashi Murayama,Nagomi Kurebayashi
Takashi Murayama
The ryanodine receptor (RyR) is a Ca2+ release channel that is present in the sarcoplasmic reticulum and endoplasmic reticulum (ER) and that plays a central role in excitation-contraction coupling in skeletal and cardiac muscle. Hyperactiva...
Chang Liu,Tianbo Li,Jun Chen
Chang Liu
Due to their important physiological functions, ion channels are key therapeutic targets for a variety of disorders. However, electrophysiological assessment of ion channel activity is technically challenging and has been a bottleneck in th...
A Simple Assay to Evaluate the Function of Human Connexin Hemichannels Expressed in Escherichia coli that Can Be Used for Drug Discovery and Mutant Analysis [0.03%]
一种简单的测定方法：评估大肠杆菌中人类连接蛋白半通道功能的药物发现和突变分析测定法
Mariana C Fiori,Luis G Cuello,Guillermo A Altenberg
Mariana C Fiori
Abnormally increased activity of connexin hemichannels contributes to cell damage in many disorders, including deafness, stroke, and cardiac infarct, and therefore hemichannels constitute a potentially important therapeutic target. Unfortun...
Fernando Aranda,Miguel Barajas,Eduardo Huarte
Fernando Aranda
Chimeric antigen receptor (CAR) T-cell therapy against tumor antigens involves a recombinant immunoreceptor that combines an antibody-derived targeting fragment with signaling domains capable of activating T cells and fusion of this recepto...
John A Beutler
John A Beutler
Many natural products have been used as drugs for the treatment of diverse indications. Although most U.S. pharmaceutical companies have reduced or eliminated their in-house natural-product research over the years, new approaches for compou...
Monika D Scuron,Brittany Fay,Julian Oliver et al.
Monika D Scuron et al.
The non-obese diabetic (NOD) mouse model is the most widely described and validated method for investigating human primary Sjögren's syndrome (SS) and represents a useful model for translational studies. However, the systemic disease manif...
Cerebral Open Flow Microperfusion to Monitor Drug Transport Across the Blood-Brain Barrier [0.03%]
用于监测药物通过血脑屏障的输送的脑开放流动微透析技术
Joanna Hummer,Thomas Altendorfer-Kroath,Thomas Birngruber
Joanna Hummer
Drugs for neurological diseases have to cross the blood-brain barrier (BBB) to induce their therapeutic effect. In vivo drug quantification in the brain is challenging, because invasive methods damage the BBB and measurement results may be ...
Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening [0.03%]
线粒体通透转换孔在疾病表型和药物筛选中的作用研究
Gauri Bhosale,Michael R Duchen
Gauri Bhosale
Mitochondria act as 'sinks' for Ca2+ signaling, with mitochondrial Ca2+ uptake linking physiological stimuli to increased ATP production. However, mitochondrial Ca2+ overload can induce a cellular catastrophe by opening of the mitochondrial...
Determination of Dermal Pharmacokinetics by Microdialysis Sampling in Rats [0.03%]
大鼠皮肤微透析取样及其药动学确定研究
Nivea M F Voelkner,Alexander Voelkner,Hartmut Derendorf
Nivea M F Voelkner
The evaluation of absorption and availability at the site of action of a drug candidate is an important element of drug discovery and development, as clinical response is a function of the bioavailability of the active agent and its continu...