Chris Shelley
Chris Shelley
This is a companion to UNIT 11.16: Single-Channel Recording of Glutamate Receptors. Described here are techniques for analyzing single-channel currents recorded from glutamate receptors to characterize their properties. In addition, issues ...
Chris Shelley
Chris Shelley
Highlighted in this unit are issues that should be considered when recording glutamate receptors at the single-channel level, including some commonly encountered problems and their remedies. "UNIT 11.17, Single-Channel Analysis of Glutamate...
Guinea pig models of asthma [0.03%]
哮喘的小鼠模型
Alice E McGovern,Stuart B Mazzone
Alice E McGovern
Described in this unit are methods for establishing guinea pig models of asthma. Sufficient detail is provided to enable investigators to study bronchoconstriction, cough, airway hyperresponsiveness, inflammation, and remodeling. ...
Sergey A Vishnivetskiy,Xuanzhi Zhan,Qiuyan Chen et al.
Sergey A Vishnivetskiy et al.
Purified arrestin proteins are necessary for biochemical, biophysical, and crystallographic studies of these versatile regulators of cell signaling. Described herein is a basic protocol for arrestin expression in E. coli and purification of...
Vsevolod V Gurevich,Eugenia V Gurevich
Vsevolod V Gurevich
Arrestins are characterized by their ability to selectively bind active, phosphorylated GPCRs and suppress (arrest) receptor coupling to G proteins. Nonvisual arrestins are also signaling proteins in their own right, activating a variety of...
Modeling human liver cancer heterogeneity: virally induced transgenic models and mouse genetic models of chronic liver inflammation [0.03%]
人肝癌异质性的建模：病毒诱导的转基因模型和慢性肝炎的小鼠遗传学模型
Marc Ringelhan,Florian Reisinger,Detian Yuan et al.
Marc Ringelhan et al.
In addition to being the most common primary liver cancer, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death in humans. Treatment options are limited for this chemoresistant cancer, with liver transplant...
Overview of pharmacokinetics [0.03%]
药代动力学概论
Lisa D Aimone,Inés A M de Lannoy
Lisa D Aimone
In addition to having selective potency against the molecular target(s), a compound must be able to reach its intended site of action in vivo in sufficient quantity and for the appropriate duration of time to exert a biological effect. The ...
Alexandra L Bey,Yong-Hui Jiang
Alexandra L Bey
This overview describes many well characterized mouse models of autism spectrum disorders (ASDs). Mouse models considered here were selected because they are examples of genetically engineered models where human genetic evidence supports a ...
The DEN and CCl4 -Induced Mouse Model of Fibrosis and Inflammation-Associated Hepatocellular Carcinoma [0.03%]
DEN和CCl4诱导的小鼠肝纤维化及炎性肝细胞癌模型
Takeki Uehara,Igor P Pogribny,Ivan Rusyn
Takeki Uehara
Human hepatocellular carcinoma (HCC) develops most often as a complication of fibrosis or cirrhosis. While most human studies of HCC provide crucial insights into the molecular signatures of HCC, seldom do they address the etiology of HCC. ...
In vivo electrophysiological recording techniques for the study of neuropathic pain in rodent models [0.03%]
用于啮齿类动物模型神经病理性疼痛研究的在体电生理记录技术
Fei-Yue Zhao,Ross Jeggo,Haifeng Wei et al.
Fei-Yue Zhao et al.
Neuropathic pain develops following nerve injury, and is a chronic pain syndrome that can persist long after repair of a wound or removal of the neurological insult. This condition remains poorly treated, not least because of a lack of mech...