Targeted Protein Degradation Phenotypic Studies Using HaloTag CRISPR/Cas9 Endogenous Tagging Coupled with HaloPROTAC3 [0.03%]
基于HaloTag的CRISPR/Cas9内源性标记结合HaloPROTAC3靶向降解的表型研究
Elizabeth A Caine,Sarah D Mahan,Rebecca L Johnson et al.
Elizabeth A Caine et al.
To assess the role of a protein, protein loss phenotypic studies can be used, most commonly through mutagenesis RNAi or CRISPR knockout. Such studies have been critical for the understanding of protein function and the identification of put...
Preclinical Models for Studying the Impact of Macrophages on Cancer Cachexia [0.03%]
癌性恶病质小鼠模型中巨噬细胞对肌肉损失和能量代谢的调节作用研究进展
Spas Dimitrov Markov,Daisy Gonzalez,Kamiya Mehla
Spas Dimitrov Markov
Cancer-associated cachexia is defined by loss of weight and muscle mass, and by the potential loss of adipose tissue accompanied by insulin resistance and increased resting energy expenditure. Cachexia is most prevalent in pancreatic cancer...
In Vitro Permeation Test (IVPT) for Pharmacokinetic Assessment of Topical Dermatological Formulations [0.03%]
体外透皮实验（IVPT）在评价局部皮肤用药的药代动力学中的应用
Leandro L Santos,Nathaniel J Swofford,Brandon G Santiago
Leandro L Santos
In vitro assessment of topical (dermal) pharmacokinetics is a critical aspect of the drug development process for semi-solid products (e.g., solutions, foams, sprays, creams, gels, lotions, ointments), allowing for informed selection of new...
A Localized Aldara (5% Imiquimod)-Induced Psoriasiform Dermatitis Model in Mice Using Finn Chambers [0.03%]
使用Finn Chambers在小鼠中建立局部Aldara(5% Imiquimod)诱导的类似银屑病的皮炎模型
Szabina Horváth,Ágnes Kemény,Erika Pintér et al.
Szabina Horváth et al.
The expanding number of research studies utilizing the imiquimod-induced psoriasiform dermatitis model attests to the usefulness of this procedure. Advantages of this model include rapid development of the skin response and cost-effectivene...
Juan Fu,Young J Kim
Juan Fu
With the rapid approval of immune checkpoint inhibitors for lung, melanoma, breast, genitourinary, and hematological malignancies, the hematopoietic cells in the tumor microenvironment (TME) are now considered an important, if not essential...
Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents [0.03%]
用于评估新型治疗药物的免疫肿瘤学研究的人源化小鼠模型的建立：外周血单个核细胞或脐带血CD34阳性造血干细胞来源
Bhavna Verma,Amy Wesa
Bhavna Verma
The clinical success of immune checkpoint modulators and the development of next-generation immune-oncology (IO) agents underscore the need for robust preclinical models to evaluate novel IO therapeutics. Human immune system (HIS) mouse mod...
Screening Assay Protocols Targeting the Nav1.7 Channel Using Qube High-Throughput Automated Patch-Clamp System [0.03%]
用于Qube高通量自动膜片钳系统靶向Nav1.7通道的筛选试验方案
Binbin Qian,Sung Hoon Park,Weifeng Yu
Binbin Qian
This article describes the basic procedure for setting up the screening protocol and recording data for Nav1.7 on a Qube automated patch-clamp system. Three protocols along with step-by-step details are provided. First, we describe a protoc...
Electrophysiological Studies of GABAA Receptors Using QPatch II, the Next Generation of Automated Patch-Clamp Instruments [0.03%]
采用新一代自动化膜片钳检测仪QPatch II研究GABAA受体的电生理学特性
Melanie Schupp,Sung Hoon Park,Binbin Qian et al.
Melanie Schupp et al.
Ligand-gated ion channels (LGICs) are a group of diverse ion channels that are gated by ligands and play important roles in normal physiological and pathological conditions. Many of them are drug targets that have been pursued, are being pu...
Studying Nicotinic Acetylcholine Receptors Using the IonFlux™ Microfluidic-Based Automated Patch-Clamp System with Continuous Perfusion and Fast Solution Exchange [0.03%]
采用IonFlux微流体膜片钳系统研究烟碱型乙酰胆碱受体
Ali Yehia,Haiyang Wei
Ali Yehia
Automated patch-clamp (APC) systems have become indispensable tools of drug-discovery programs by allowing high-throughput electrophysiology-based screening of ion channel compounds. The recent development and introduction of microfluidics-...
Fluorescent Western Blotting: High Sensitivity Detection of Multiple Targets [0.03%]
荧光西方墨点法-多种目标高灵敏度检测
Tom Berkelman
Tom Berkelman
Western blotting with fluorescence detection offers the possibility of detecting multiple targets simultaneously on a single blot. Primary antibodies are increasingly available from multiple hosts, and there are now a wide variety of dye la...