Mark A Exley,S Brian Wilson,Steven P Balk
Mark A Exley
This unit details methods for the isolation, in vitro expansion, and functional characterization of human iNKT cells. The term 'iNKT' derives from the fact that a large fraction of murine and some human NK marker+ T cells ('NKT') recognize ...
Nicolas Bouladoux,Oliver J Harrison,Yasmine Belkaid
Nicolas Bouladoux
Citrobacter rodentium is a murine mucosal pathogen used as a model to elucidate the molecular and cellular pathogenesis of infection with two clinically important human gastrointestinal pathogens, enteropathogenic Escherichia coli (EPEC) an...
A Real-Time Cytotoxicity Assay as an Alternative to the Standard Chromium-51 Release Assay for Measurement of Human NK and T Cell Cytotoxic Activity [0.03%]
实时细胞毒性测定作为标准51铬释放测定法的一种替代方法，用于测量人NK和T细胞的细胞毒性活性
Julien Fassy,Kyriaki Tsalkitzi,Maria Goncalves-Maia et al.
Julien Fassy et al.
This unit describes the monitoring and quantification of cellular cytotoxicity using a non-radioactive and real-time cytotoxic assay. The extent of target-cell lysis is monitored over time by imaging and quantifying live fluorescent target ...
James McCluskey,Carmel Kanaan,Mary Diviney
James McCluskey
This overview presents nomenclature and serology information on human leucocyte antigens, or HLA molecules, which are encoded by a cluster of genes linked on the short arm of chromosome 6. This region is known as the major histocompatibilit...
Universal Precautions: Necessary Safety Procedures When Handling Human Blood, Body Fluids, and Specimens [0.03%]
标准预防措施：处理人血、体液及标本时的必要安全规程
Barbara E Bierer
Barbara E Bierer
Universal precautions are observed whenever handling human blood, body fluids, or specimens as a means of preventing exposure to blood-borne pathogens. This appendix outlines safety procedures to follow whenever undertaking research activit...
Evida A Dennis,Tanya O Robinson,Lesley E Smythies et al.
Evida A Dennis et al.
Monocytes and macrophages play fundamental roles in defense against microbes, clearance of senescent and dead cells, and immunoregulation. Although blood monocytes are the source of intestinal macrophages in the developed mucosal immune sys...
Venipuncture Procedures: Sources of Human Peripheral Blood Cells, Serum, and Plasma [0.03%]
静脉穿刺取血：人类外周血细胞、血清和血浆的来源
Barbara E Bierer,Warren Strober
Barbara E Bierer
Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 ...
Tess Melinda Brodie,Vinko Tosevski
Tess Melinda Brodie
Mass cytometry is an analytical technology that combines the sample preparation workflow typical of flow cytometry and the detection capacity of atomic mass spectroscopy, allowing for highly multiplexed measurements of protein or nucleic ac...
Kathy D McCoy,Markus B Geuking,Francesca Ronchi
Kathy D McCoy
Mouse models are used extensively to study human health and to investigate the mechanisms underlying human disease. In the past, most animal studies were performed without taking into consideration the impact of the microbiota. However, the...
Zofia Maciorowski,Pratip K Chattopadhyay,Paresh Jain
Zofia Maciorowski
Multicolor flow cytometry is a rapidly evolving technology that uses multiple fluorescent markers to identify and characterize cellular subpopulations of interest, allowing rapid analysis on tens of thousands of cells per second, with the p...