Isolation of Murine Skin Resident and Migratory Dendritic Cells via Enzymatic Digestion [0.03%]
通过酶消化法分离小鼠皮肤驻留和迁移型树突状细胞
Sakeen W Kashem,Daniel H Kaplan
Sakeen W Kashem
Dendritic cells (DCs) are a highly specialized subset of professional antigen-presenting cells (APCs) that reside in peripheral and lymphoid tissues. DCs capture antigen in the periphery and migrate to the lymph node where they prime naïve...
Leukocyte Isolation from Brain, Spinal Cord, and Meninges for Flow Cytometric Analysis [0.03%]
脑、脊髓和硬脑膜白细胞的分离以进行流式分析
Monica Manglani,Selamawit Gossa,Dorian B McGavern
Monica Manglani
A continual dialogue exists between the central nervous system (CNS) and immune system that contributes to neural homeostasis as well as protection from microbes, repair following damage, autoimmune disease, and neurodegeneration. Character...
Andreas B den Hartigh,Susan L Fink
Andreas B den Hartigh
Pyroptosis is a form of programmed pro-inflammatory cell death that plays a protective role in the host response to infection, but can also promote pathogenic inflammation. Pyroptosis is mediated by the cysteine protease, caspase-1. Caspase...
Gnotobiotic and Conventional Mouse Systems to Support Microbiota Based Studies [0.03%]
无菌和常规小鼠系统支持微生物群研究
Richard Lavin,Nicholas DiBenedetto,Vladimir Yeliseyev et al.
Richard Lavin et al.
Animal models are essential to dissect host-microbiota interactions that impact health and the development of disease. In addition to providing pre-clinical models for the development of novel therapeutics and diagnostic biomarkers, mouse s...
Meningeal whole mount preparation and characterization of neural cells by flow cytometry [0.03%]
脑膜组织的制备及神经细胞的特性分析
Antoine Louveau,Anthony J Filiano,Jonathan Kipnis
Antoine Louveau
Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous imm...
Monocyte and Neutrophil Isolation, Migration, and Phagocytosis Assays [0.03%]
单核细胞和中性粒细胞的分离、迁移及吞噬实验方法
Alexander Maini,Juliet R Foote,Richard Hayhoe et al.
Alexander Maini et al.
This article describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell phagocytosis, migration, and polarization. The method employed here for the isolation of naive phagocytes overcom...
Lisette Krabbendam,Maho Nagasawa,Hergen Spits et al.
Lisette Krabbendam et al.
Innate lymphoid cells (ILCs) are innate immune cells of lymphoid origin that have important effector and regulatory functions in the first line of defense against pathogens, but also regulate tissue homeostasis, remodeling, and repair. Thei...
Quantifying Microglial Phagocytosis of Apoptotic Cells in the Brain in Health and Disease [0.03%]
定量分析脑内微胶质细胞在健康和疾病状态下对凋亡细胞的吞噬作用
Sol Beccari,Irune Diaz-Aparicio,Amanda Sierra
Sol Beccari
Apoptosis is a ubiquitous process occurring in the brain under both physiological and pathological conditions. The central nervous system (CNS) requires an active and efficient clean-up system to prevent the spillover of intracellular conte...
Ada M Kruisbeek
Ada M Kruisbeek
Barbara E Bierer
Barbara E Bierer