Yuelin Kong,Yi Jing,Maria Bettini
Yuelin Kong
The ability to express and study a single T cell receptor (TCR) in vivo is an important aspect of both basic and translational immunological research. Traditionally, this was achieved by using TCR transgenic mice. In the past decade, a more...
Peptide:MHCII Tetramer-Based Cell Enrichment for the Study of Epitope-Specific CD4+ T Cells [0.03%]
基于肽:MHCII四聚体的细胞富集技术研究表位特异性CD4+T细胞
Dmitri I Kotov,Marc K Jenkins
Dmitri I Kotov
Epitope-specific CD4+ T cells can be labeled in complex cell mixtures from secondary lymphoid organs with fluorophore-labeled peptide:major histocompatibility complex class II (p:MHCII) tetramers and then detected by flow cytometry. Magneti...
Identification of Group 2 Innate Lymphoid Cells in Mouse Lung, Liver, Small Intestine, Bone Marrow, and Mediastinal and Mesenteric Lymph Nodes [0.03%]
小鼠肺、肝脏、小肠、骨髓及纵隔和肠系膜淋巴结中第2组固有淋巴样细胞的鉴定
Mónica Romera-Hernández,Laura Mathä,Catherine A Steer et al.
Mónica Romera-Hernández et al.
Innate lymphoid cells (ILCs) are a heterogeneous family of lymphocytes that populate barrier and non-barrier tissues. ILCs regulate immune responses to pathogens and commensals but also sustain metabolic homeostasis, tissue remodeling after...
Ulrike Steffen,Fabian T Andes,Georg Schett
Ulrike Steffen
Osteoclasts are the only bone-resorbing cells in the body. Together with bone-forming osteoblasts, they are responsible for bone homeostasis and constant bone remodeling. Aberrant activation of osteoclasts leads to bone loss, as seen in pos...
Kate A Markey,Kate H Gartlan
Kate A Markey
This unit describes methods for quantifying phagocytosis and imaging the immunological synapse between T cells and antigen-presenting cells (APCs), with both techniques delivering valuable information about APC function. These aspects of AP...
IL-23- and Imiquimod-Induced Models of Experimental Psoriasis in Mice [0.03%]
小鼠IL-23和咪喹莫特诱导的实验性银屑病模型
Tej Pratap Singh,Howard H Zhang,Samuel T Hwang et al.
Tej Pratap Singh et al.
Genome-wide association studies have found that polymorphisms in genes for IL-23 and its receptor are important in psoriasis, and blocking IL-23 is an effective therapy in the disease. The use of Aldara™ , a cream that contains the TLR7 an...
Christopher J Bohlen,F Chris Bennett,Mariko L Bennett
Christopher J Bohlen
Microglia represent 5-10% of cells in the central nervous system and contribute to the development, homeostasis, injury, and repair of neural tissues. As the tissue-resident macrophages of the central nervous system, microglia execute core ...
Jeff Skinner
Jeff Skinner
Statistical tests and graphs are an important part of any immunological research publication or presentation, but not all immunologists have the statistical expertise to choose the best methods to evaluate and represent their experiments. T...
Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells [0.03%]
CRISPR/Cas9基因敲除在原代T细胞中采用核糖核蛋白转染方法
Soyoung A Oh,Akiko Seki,Sascha Rutz
Soyoung A Oh
CRISPR/Cas9 has enabled the rapid and efficient generation of gene knockouts across various cell types of several species. T cells are central players in adaptive immune responses. Gene editing in primary T cells not only represents a valua...
Efficient CRISPR/Cas9-Mediated Mutagenesis in Primary Murine T Lymphocytes [0.03%]
高效的一步法CRISPR/Cas9介导的原代小鼠T淋巴细胞基因打靶技术
Bonnie Huang,Kristoffer Haurum Johansen,Pamela L Schwartzberg
Bonnie Huang
The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling, and function. Manipulation of T cell clones and primary T cells has been accomplished primarily through...